Hes1-deficient mice show precocious differentiation of Paneth cells in the small intestine.
Hes1-deficient mice show precocious differentiation of Paneth cells in the small intestine.
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DOI：
10.1016/j.bbrc.2004.12.174
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发表时间：
2005-03
影响因子：
3.1
通讯作者：
Katsumasa Suzuki;H. Fukui;T. Kayahara;M. Sawada;H. Seno;H. Hiai;R. Kageyama;H. Okano;T. Chiba
中科院分区：
文献类型：
--
作者：
Katsumasa Suzuki;H. Fukui;T. Kayahara;M. Sawada;H. Seno;H. Hiai;R. Kageyama;H. Okano;T. Chiba
我们之前的研究表明，Hes1在Paneth细胞上方的推定上皮干细胞和Paneth细胞之间的隐窝基柱状细胞中都有表达，而Hes1在Paneth细胞中完全不存在。本研究利用Hes1基因敲除（KO）新生小鼠（P0）来阐明Hes1在Paneth细胞分化中的作用。电镜观察显示，Hes1-KO型P0小鼠绒毛间区有明显的含胞质颗粒的细胞，而野生型（WT） P0小鼠绒毛间区没有这些细胞。在Hes1-KO P0小鼠中，仅存在于Paneth细胞的cryptdins基因表达均较WT P0小鼠增强。免疫组化结果显示，Hes1-KO P0小鼠小肠上皮溶菌酶阳性细胞和隐蛋白4阳性细胞数量均高于WT P0小鼠。因此，Hes1似乎在小肠Paneth细胞分化中具有抑制作用。
We have previously shown that Hes1 is expressed both in putative epithelial stem cells just above Paneth cells and in the crypt base columnar cells between Paneth cells, while Hes1 is completely absent in Paneth cells. This study was undertaken to clarify the role of Hes1 in Paneth cell differentiation, using Hes1-knockout (KO) newborn (P0) mice. Electron microscopy revealed premature appearance of distinct cells containing cytoplasmic granules in the intervillous region in Hes1-KO P0 mice, whereas those cells were absent in wild-type (WT) P0 mice. In Hes1-KO P0 mice, the gene expressions of cryptdins, exclusively present in Paneth cells, were all enhanced compared with WT P0 mice. Immunohistochemistry demonstrated increased number of both lysozyme-positive and cryptdin-4-positive cells in the small intestinal epithelium of Hes1-KO P0 mice as compared to WT P0 mice. Thus, Hes1 appears to have an inhibitory role in Paneth cell differentiation in the small intestine.