Experimental herpesvirus keratitis in the rabbit: topical versus intrastromal infection routes.
Experimental herpesvirus keratitis in the rabbit: topical versus intrastromal infection routes.
复制标题
兔子实验性疱疹病毒角膜炎：局部感染途径与基质内感染途径。
DOI：
10.1159/000265266
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发表时间：
1983
影响因子：
2.1
通讯作者：
Dethlefs,BA
中科院分区：
文献类型：
--
作者：
Meyers-Elliott,RH;Chitjian,PA;Dethlefs,BA
在临床上比较了单纯疱疹病毒1型HF株感染兔角膜的基质内和局部途径，以确定哪种感染途径是最佳的角膜水肿和深层基质角膜炎模型，以便我们进一步研究感染动物的免疫应答如何影响临床疾病的表达。此外，通过病毒学和电子显微镜免疫细胞化学研究监测病毒清除和作为免疫刺激的病毒抗原的持久性。我们发现，局部感染是更可取的间质内感染途径提出间质疾病，因为它导致上皮疾病的发病率较高，前房参与较少。局部感染途径模型在研究细胞介导的抗HSV感染细胞的免疫应答中具有重要价值。
Intrastromal and topical routes of infection of rabbit corneas with the HF strain of herpes simplex virus type 1 were compared clinically to determine which route of infection would present the best model of disciform edema and of deep stromal keratitis for our further studies on how the immune response of the infected animals may influence the expression of clinical disease. In addition, virus clearing and persistence of viral antigens as immunologic stimuli were monitored by virologic and electron microscopic immunocy-tochemical studies. We found topical infection to be preferable to the intrastromal infection route for presenting stromal disease in that it resulted in a higher incidence of epithelial disease with less anterior chamber involvement. The topical infection route model should be valuable in studies of cell-mediated immune response against HSV-infected cells.