Restoration of vision after transplantation of photoreceptors.
Restoration of vision after transplantation of photoreceptors.
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DOI：
10.1038/nature10997
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发表时间：
2012-05-03
期刊：
影响因子：
64.8
通讯作者：
Ali, R. R.
中科院分区：
文献类型：
--
作者：
Pearson, R. A.;Barber, A. C.;Rizzi, M.;Hippert, C.;Xue, T.;West, E. L.;Duran, Y.;Smith, A. J.;Chuang, J. Z.;Azam, S. A.;Luhmann, U. F. O.;Benucci, A.;Sung, C. H.;Bainbridge, J. W.;Carandini, M.;Yau, K. -W.;Sowden, J. C.;Ali, R. R.
细胞移植是治疗因光感受器丧失引起的失明的潜在策略。虽然移植的视杆细胞前体细胞能够迁移到成年视网膜并分化获得成熟感光细胞的特殊形态特征，但根本问题仍然是感光细胞移植是否真的能改善视力。在这里，我们提供了成年Gnat 1 −/−小鼠感光细胞移植后功能性视杆介导的视觉的证据，这些小鼠缺乏视杆功能，是先天性静止性夜盲症的模型。我们发现，移植杆前体形成经典的三联突触连接与受体视网膜中的二阶双极和水平细胞。新整合的光感受器细胞具有与成年野生型光感受器相似的光响应性和微光动力学。通过在暗视条件下使用内在成像，我们证明了由移植杆产生的视觉信号被投射到更高的视觉区域，包括V1。此外，这些细胞能够在暗视条件下驱动Gnat 1 −/−小鼠的视动头部跟踪和视觉引导行为。总之，这些结果证明了光感受器移植作为视网膜变性后恢复视力的治疗策略的可行性。
Cell transplantation is a potential strategy for treating blindness caused by the loss of photoreceptors. Although transplanted rod-precursor cells are able to migrate into the adult retina and differentiate to acquire the specialized morphological features of mature photoreceptor cells, the fundamental question remains whether transplantation of photoreceptor cells can actually improve vision. Here we provide evidence of functional rod-mediated vision after photoreceptor transplantation in adultGnat1−/−mice, which lack rod function and are a model of congenital stationary night blindness. We show that transplanted rod precursors form classic triad synaptic connections with second-order bipolar and horizontal cells in the recipient retina. The newly integrated photoreceptor cells are light-responsive with dim-flash kinetics similar to adult wild-type photoreceptors. By using intrinsic imaging under scotopic conditions we demonstrate that visual signals generated by transplanted rods are projected to higher visual areas, including V1. Moreover, these cells are capable of driving optokinetic head tracking and visually guided behaviour in theGnat1−/−mouse under scotopic conditions. Together, these results demonstrate the feasibility of photoreceptor transplantation as a therapeutic strategy for restoring vision after retinal degeneration.
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外部极限膜的药理破坏会导致移植光感受器前体的视网膜整合增加。
影响因子：
3.4
作者：
West, E. L.;Pearson, R. A.;Tschernutter, M.;Sowden, J. C.;MacLaren, R. E.;Ali, R. R.
通讯作者：
Ali, R. R.
影响因子：
3.4
作者：
Berson, Eliot L.
通讯作者：
Berson, Eliot L.
DOI：
10.1073/pnas.250478897
发表时间：
2000-12-05
影响因子：
11.1
作者：
Calvert, PD;Krasnoperova, NV;Lem, J
通讯作者：
Lem, J
影响因子：
1.8
作者：
Prusky, GT;West, PWR;Douglas, RM
通讯作者：
Douglas, RM
影响因子：
4.4
作者：
Maeda, T;Lem, J;Haeseleer, F
通讯作者：
Haeseleer, F