Evaluation of propolis (II): Effects of Brazilian and Chinese propolis on histamine release from rat peritoneal mast cells induced by compound 48/80 and concanavalin A
Evaluation of propolis (II): Effects of Brazilian and Chinese propolis on histamine release from rat peritoneal mast cells induced by compound 48/80 and concanavalin A
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DOI：
10.1248/bpb.21.723
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发表时间：
1998-07-01
影响因子：
2
通讯作者：
Satoh, T
中科院分区：
文献类型：
--
作者：
Miyataka, H;Nishiki, M;Satoh, T
为了建立蜂胶的生物学评价方法并揭示其抗过敏作用，研究了巴西蜂胶、中国蜂胶和日本蜂胶的乙醇提取物(EA-ET)和水提物(WA-WT)对化合物48/80和刀豆蛋白A(ConA)诱导的组胺释放的影响，并考察了这些提取物对组胺释放的抑制活性与E-1 cm(1%)的关系。结果表明：0.003~0.01%乙醇提取物和0.03~0.1%水提取物对48/80和刀豆蛋白A诱导的组胺释放均有抑制作用，且前者的抑制作用比后者强10倍以上，说明乙醇提取物和水提取物均具有抗过敏作用：2)大部分乙醇和水提取物对这两种组胺释放剂诱导的组胺释放具有浓度依赖性反应；3)河北产0.003%EM、四川产EP、浙江产EQ和安徽产ER对中国体内组胺释放的抑制作用弱于0.01%对应提取物，而巴西蜂胶0.001%ED-EH、中国产EM、河南产EN和EP-ER对刀豆蛋白A诱导的组胺释放的促进作用均在10%以上，应慎用；4)仅0.03-0.1%的中国蜂胶水提物对ConA诱导的组胺释放的抑制作用与它们的E-1 cm(1%)值有很好的相关性，5)从蜂胶提取物的抑制效力与它们的E-1 cm(1%)值的关系来看，推测蜂胶中含有一种具有抗过敏作用的难于水溶的非黄酮类化合物；6)为了准确评价蜂胶的抗过敏作用，测定蜂胶提取物抑制组胺释放活性的生物法明显优于物理化学法。
To establish a biological method for evaluating propolis and to reveal their anti-allergic action, the effects of the ethanol and water extracts (EA-ET and WA-WT, respectively) from Brazilian, Chinese and Japanese propolis on the histamine release induced by compound 48/80 and concanavalin A (Con A) were investigated, The relation between the inhibitory activities of these extracts on the histamine release and their E-1cm(1%) values were also examined. As a result, the following was found: 1) 0.003-0.01% ethanol and 0.03-0.1% water extracts inhibited the histamine release induced by compound 48/80 and Con A, and the inhibitory potencies of the former extracts were more than 10 times stronger than those of the latter extracts, making it clear that both the ethanol and water extracts possesse an anti-allergic action; 2) most of the ethanol and water extracts responded to the histamine release induced by both the histamine releasers in a concentration-dependent manner; 3) the inhibitory activities of 0.003% EM from Hebei Province, EP from Sichuan Province, EQ from Zhejiang Province and ER from Anhui Province in China were weaker than those of 0.01% corresponding extracts, whereas 0.001% ED-EH from Brazilian propolis, EM, EN from Henan Province in China and EP-ER promoted the Con A-induced histamine release of more than 10%, suggesting that such extracts must be carefully given to humans; 4) the inhibitory potencies of only 0.03-0.1% water extracts from Chinese propolis on the Con A-induced histamine release related excellently with their E-1cm(1%) values; 5) from the results of the relation between the inhibitory potencies of the propolis extracts and their E-1cm(1%) values, it was suggested that an unknown compound, being a poorly water-soluble compound which is a non-flavonoid, with an anti-allergic action is contained in propolis; 6) to precisely evaluate the anti-allergic action of the propolis, the biological method, which measures the inhibitory activities of the propolis extracts on histamine release, was markedly superior to the physicochemical method.