Serodiagnosis of Salmonella enterica serovar typhi and S-enterica serovars Paratyphi A, B and C human infections
Serodiagnosis of Salmonella enterica serovar typhi and S-enterica serovars Paratyphi A, B and C human infections
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DOI：
10.1099/jmm.0.47197-0
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发表时间：
2007-09-01
影响因子：
3
通讯作者：
Thomas, E. John
中科院分区：
文献类型：
--
作者：
Chart, Henrik;Cheasty, Thomas;Thomas, E. John
本研究的目的是评估用于检测伤寒沙门氏菌和甲型、乙型副伤寒沙门氏菌和丙型副伤寒沙门氏菌的脂多糖和鞭毛抗原以及伤寒沙门氏菌和丙型副伤寒沙门氏菌Vi荚膜多糖的人血清抗体的免疫测定法。总共使用了330份血清；这些样本来自 15 名伤寒沙门氏菌培养呈阳性的患者和 15 名健康对照者，以及提交给肠道病原体实验室进行沙门氏菌血清诊断的 300 份血清。通过 SDS-PAGE/免疫印迹，来自培养阳性患者的所有 15 份血清均具有针对 9,12 LPS 抗原的血清抗体，其中 10 份血清具有针对 V 鞭毛抗原的抗体。在 300 份参考血清中，22 份具有针对 9,12 LPS 抗原的抗体，一份针对 1,4,5,12 LPS 抗原，12 份针对 6,7 LPS 抗原。只有两种血清具有针对鞭毛抗原的抗体，其中一种与 V 结合，另一种与 V 抗原结合。开发了一种 ELISA，成功检测了针对 Vi 荚膜多糖的血清抗体，但由于针对 Vi 的血清抗体产生的动力学，这些抗体在伤寒沙门氏菌和丙型副伤寒沙门氏菌急性感染的血清诊断中的价值可能有限。此处描述的免疫测定法提供了检测针对 LPS、鞭毛和丙型副伤寒沙门氏菌的血清抗体的灵敏方法。 Vi 抗原伤寒沙门氏菌和副伤寒沙门氏菌，并构成了用于筛选血清的 Widal 测定的可行替代品。这里描述的沙门氏菌血清诊断方案是位于英国科林代尔肠道病原体实验室的健康保护局国家沙门氏菌参考中心使用的新标准操作程序。
The aim of this study was to evaluate an immunoassay for the detection of human serum antibodies to the LPS and flagellar antigens of Salmonella Typhi and Salmonella Paratyphi A, B and C, and to the Vi capsular polysaccharide of S. Typhi and S. Paratyphi C. A total of 330 sera were used; these originated from 15 patients who were culture-positive for S. Typhi and 15 healthy controls, together with 300 sera submitted to the Laboratory of Enteric Pathogens for Salmonella serodiagnosis. By SDS-PAGE/immunoblotting, all 15 sera from culture-positive patients had serum antibodies to the 9,12 LPS antigens and 10 had antibodies to the V flagellar antigens. Of the 300 reference sera, 22 had antibodies to the 9,12 LPS antigens, one to the 1,4,5,12 LPS antigens and 12 to the 6,7 LPS antigens. Only two sera had antibodies to flagellar antigens, one of which bound to the V and the other to the V antigen. An ELISA was developed that successfully detected serum antibodies to the Vi capsular polysaccharides, but because of the kinetics of serum antibody production to the Vi, these antibodies may be of limited value in the serodiagnosis of acute infection with S. Typhi and S. Paratyphi C. The immunoassays described here provide a sensitive means of detecting serum antibodies to the LPS, flagellar and Vi antigens of S. Typhi and S. Paratyphi, and constitute a viable replacement for the Widal assay for the screening of sera. The Salmonella serodiagnosis protocols described here are the new standard operating procedures used by the Health Protection Agency's National Salmonella Reference Centre based in the Laboratory of Enteric Pathogens, Colindale, UK.