Centrifugo-pneumatic valve for metering of highly wetting liquids on centrifugal microfluidic platforms
Centrifugo-pneumatic valve for metering of highly wetting liquids on centrifugal microfluidic platforms
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DOI：
10.1039/b914415c
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
von Stetten, Felix
中科院分区：
文献类型：
--
作者：
Mark, Daniel;Metz, Tobias;von Stetten, Felix
We designed and experimentally validated a new type of passive valve for centrifugal microfluidic platforms. A liquid column entering an unvented receiving chamber is stopped by the counter-pressure of compressed air. This valve opens under defined conditions at high centrifugal frequencies at which the interface between liquid and air becomes unstable and enables a phase exchange, forwarding the liquid. Burst frequencies of the valve were determined for liquids typically used in biochemical assays: pure water, water with detergent concentrations between 0.01 and 10%, and pure ethanol. Burst frequencies between 8.5 +/- 0.6 and 27.9 +/- 2.0 Hz were measured for different surface tensions. The burst frequencies can be tuned by simple geometrical changes in the valving structure. The valve does not require ultra-precise structures or local surface modifications and is therefore ideal for low-cost microfluidic polymer disks. Potential applications are in the field of multiparameter and panel analysis, such as PCR-genotyping.
