Outer membrane vesicles derived from Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection
Outer membrane vesicles derived from Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection
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DOI：
10.1016/j.vaccine.2013.08.059
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发表时间：
2013-10-25
期刊：
影响因子：
5.5
通讯作者：
Hozbor, D.
中科院分区：
文献类型：
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作者：
Bottero, D.;Gaillard, M. E.;Hozbor, D.
副百日咳博德氏菌是百日咳的近亲，也能引起百日咳或百日咳。由这种相关病原体引起的病例数在过去几年中持续上升。广泛使用的细胞（wP）或非细胞（aP）百日咳疫苗对百日咳双歧杆菌几乎没有或没有效力。为了研制一种有效的百日咳疫苗，从百日咳中获得了外膜囊泡（omv）。对生成的omv（命名为OMVsBpp）进行蛋白质组学分析，证实了包括perpern在内的几种表面免疫原的存在。将所制备的OMVsBpp用于小鼠鼻内攻毒模型，观察其全身给药时的保护能力。免疫BALB/c小鼠用亚致死剂量的副百日咳杆菌攻毒。免疫组与阴性对照组之间差异有统计学意义(p
Bordetella parapertussis, a close related species of B. pertussis, can also cause the disease named pertussis or whooping cough. The number of cases caused by this related pathogen has risen sustained in the last years. The widely used cellular (wP) or acellular (aP) pertussis vaccines have little or no efficacy against B. parapertussis. In an effort to devise an effective acellular vaccine against B. parapertussis infection, outer membrane vesicles (OMVs) were obtained from B. parapertussis. Proteomic analysis of the resulting OMVs, designated OMVsBpp, evidenced the presence of several surface immunogens including pertactin. The characterized OMVsBpp were used in murine B. parapertussis intranasal challenge model to examine their protective capacity when administered by systemic route. Immunized BALB/c mice were challenged with sublethal doses of B. parapertussis. Significant differences between immunized animals and the negative control group were observed (p