Extracellular matrix does not induce the proliferation, but promotes the differentiation, of Hodgkin's cell line HDLM-1.
Extracellular matrix does not induce the proliferation, but promotes the differentiation, of Hodgkin's cell line HDLM-1.
复制标题
细胞外基质不诱导霍奇金细胞系HDLM-1的增殖，但促进其分化。
作者：
S. Hsu;X. Zhao;P. Hsu;M. Lok
将来自霍奇金细胞系HDLM-1的肿瘤细胞在细胞外基质（ECM）包被的烧瓶中培养。在24小时内，细胞经历快速分化，伴随着形态和表型变化。在HDLM-1细胞中观察到一种缓慢的、不太明显的但相似的变化，在它们用佛波酯诱导5天后。ECM诱导的细胞变为HeFi-1阴性，并在其核膜上表达抗原2 H9和1 E9。这些细胞具有点状酸性磷酸酶和酯酶活性。大约70%的ECM诱导的细胞被拉长，并有长，钝的胞质突起。这些发现，加上作者先前提出的证据，表明H-RS细胞与交错网状细胞有关。作者认为，ECM诱导的HDLM-1细胞可作为研究何杰金氏病本质和细胞谱系的重要模型。
Tumor cells from the Hodgkin's cell line HDLM-1 were cultured in extracellular matrix (ECM)-coated flasks. Within 24 hours, the cells underwent rapid differentiation, accompanied by morphologic and phenotypic changes. A slow and less prominent, but similar change was observed in HDLM-1 cells after they had been induced with phorbol ester for 5 days. The ECM-induced cells became HeFi-1-negative and expressed antigens 2H9 and 1E9 on their nuclear membranes. These cells had punctate acid-phosphatase and esterase activities. Approximately 70% of the ECM-induced cells were elongated and had long, blunt cytoplasmic projections. These findings, together with evidence the authors previously presented, indicate that H-RS cells are related to interdigitating reticulum cells. The authors believe that the ECM-induced HDLM-1 cells can be used as an important model for studies of the nature and cell lineage of Hodgkin's disease.
霍奇金病的组织化学。
DOI：
--
发表时间：
1982
期刊：
Cancer treatment reports
影响因子：
--
作者：
Beckstead,JH;Warnke,R;Bainton,DF
通讯作者：
Bainton,DF
影响因子：
11.2
作者：
D. Gospodarowicz;G. Lui;R. Gonzalez
通讯作者：
D. Gospodarowicz;G. Lui;R. Gonzalez
DOI：
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发表时间：
1986
期刊：
The American journal of pathology
影响因子：
--
作者：
Hsu,SM;Ho,YS;Li,PJ;Monheit,J;Ree,HJ;Sheibani,K;Winberg,CD
通讯作者：
Winberg,CD