IDENTIFICATION OF 2 NOVEL AMYLOID-A PROTEIN SUBSETS COEXISTING IN AN INDIVIDUAL PATIENT OF AA-AMYLOIDOSIS
IDENTIFICATION OF 2 NOVEL AMYLOID-A PROTEIN SUBSETS COEXISTING IN AN INDIVIDUAL PATIENT OF AA-AMYLOIDOSIS
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DOI：
10.1016/0925-4439(92)90068-x
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发表时间：
1992-12-10
期刊：
影响因子：
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通讯作者：
SHIRASAWA, H
中科院分区：
文献类型：
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作者：
BABA, S;TAKAHASHI, T;SHIRASAWA, H
淀粉样蛋白 A (AA) 是 AA 淀粉样变性中的主要原纤维蛋白，是前体蛋白血清淀粉样蛋白 A (SAA) 的 N 末端裂解产物。使用质谱和氨基酸测序，我们鉴定并表征了患有与类风湿性关节炎相关的 AA 淀粉样变性患者体内以淀粉样原纤维形式共同沉积的两种新型 AA 蛋白亚群。其中一个 AA 蛋白对应于 SAA2α 的位置 2-76（或 75），另一个对应于已知 SAA1 子集的位置 2-76（或 75），位置 52 或 57 除外，其中 SAA1α 具有缬氨酸和丙氨酸，SAA1β 分别在位置 52 和 57 具有丙氨酸和缬氨酸，而 AA 蛋白在两个位置都具有丙氨酸。我们的研究结果 (1) 表明，不仅一个而且两个 SAA 子集可以作为 AA 淀粉样蛋白一起沉积在单个个体中，并且 (2) 支持一种新的 SAA1 同种异型的存在，即 SAA1(52,57)Ala。
Amyloid A protein (AA), the major fibril protein in AA-amyloidosis, is an N-terminal cleavage product of the precursor protein, serum amyloid A (SAA). Using mass spectrometry and amino-acid sequencing, we identified and characterized two novel AA protein subsets co-deposited as amyloid fibrils in an patient having AA-amyloidosis associated with rheumatoid arthritis. One of the AA proteins corresponded to positions 2-76 (or 75) of SAA2alpha and the other corresponded to positions 2-76 (or 75) of known SAA1 subsets, except for position 52 or 57, where SAA1alpha has valine and alanine and SAA1beta has alanine and valine in position 52 and 57, respectively, whereas the AA protein had alanine at the both positions. Our findings (1), demonstrate that not only one but two SAA subsets could be deposited together as an AA-amyloid in a single individual and (2), support the existence of a novel SAA1 allotype, i.e., SAA1(52,57)Ala.