Tracheal reconstruction using tissue-engineered cartilage
Tracheal reconstruction using tissue-engineered cartilage
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DOI：
10.1001/archotol.130.10.1191
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发表时间：
2004-10-01
影响因子：
--
通讯作者：
Weatherly, RA
中科院分区：
文献类型：
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作者：
Grimmer, JF;Gunnlaugsson, CB;Weatherly, RA
目的：为了确定是否可以使用由PGA网、自体软骨细胞和与细胞粘附序列精氨酸-甘氨酸-天冬氨酸（RGD）共价连接的藻酸盐组成的聚乙醇酸（PGA）构建兔软骨组织工程，并研究使用PGA构建结合生物相容性气管内支架重建气管缺损的可行性。9只家兔接受了3种不同PGA结构组合的皮下植入：（1）PGA、自体软骨细胞和RGD修饰的藻酸盐;（2）PGA、自体软骨细胞和未修饰的藻酸盐;以及（3）PGA和RGD修饰的藻酸盐。其余10只动物使用阔筋膜移植物和完整的PGA结构（PGA、自体软骨细胞和RGD修饰的藻酸盐）进行前气管重建。在气管重建时，将聚-L-乳酸气管内支架放置在其中5只动物中。将气管狭窄率和死亡率与历史对照动物进行比较。组织学分析进行PGA constructs.Results：在皮下植入物中，与软骨细胞（有和没有RGD）的PGA结构表现出成熟的软骨形成在7（78%）的9只动物。在没有软骨细胞的PGA构建体中没有观察到软骨。10只接受完整PGA结构气管重建的动物中有2只存活至20周，并显示气道通畅，1只使用支架，1只未使用支架（总死亡率80%）。组织学分析显示气管重建部位有成熟软骨形成。历史对照组动物进行重建与阔筋膜单独表现出最低的整体mortals.Conclusions：软骨组织工程可以在兔使用PGA网嵌入藻酸盐包封的自体软骨细胞。用这种软骨工程方法重建气管缺损也是可能的，尽管这项研究的死亡率很高。
Objectives: To determine whether rabbit cartilage can be tissue engineered using a polyglycolic acid (PGA) construct composed of PGA mesh, autologous chondrocytes, and alginate covalently linked with the cell adhesion sequence arginine-glycine-aspartic acid (RGD), and to investigate the feasibility of reconstructing tracheal defects using the PGA construct in conjunction with a bioabsorble intratracheal stent.Methods: Nineteen New Zealand White rabbits were used. Nine rabbits underwent subcutaneous implantation of 3 different PGA construct combinations: (1) PGA, autologous chondrocytes, and RGD-modified alginate; (2) PGA, autologous chondrocytes, and unmodified alginate; and (3) PGA and RGD-modified alginate. The remaining 10 animals underwent anterior tracheal reconstruction using fascia lata grafts and the complete PGA construct (PGA, autologous chondrocytes, and RGD-modified alginate). At the time of tracheal reconstruction, a poly-L-lactic acid intratracheal stent was placed in 5 of these latter animals. Rates of tracheal stenosis and mortality were compared with those of historical control animals. Histologic analysis was performed on the PGA constructs.Results: In the subcutaneous implants, the PGA constructs made with chondrocytes (with and without RGD) demonstrated mature cartilage formation in 7 (78%) of the 9 animals. No cartilage was seen in PGA constructs made without chondrocytes. Two of the 10 animals that underwent tracheal reconstruction with the complete PGA construct survived to 20 weeks and demonstrated patent airways, 1 with a stent and 1 without a stent (80% overall mortality). Histologic analysis showed mature cartilage formation at the tracheal reconstruction site. Historical control animals that underwent reconstruction with fascia lata alone demonstrated the lowest overall mortality.Conclusions: Cartilage can be tissue engineered in rabbits using PGA mesh embedded with alginate-encapsulated autologous chondrocytes. It is also possible to reconstruct tracheal defects with this method of cartilage engineering, although the mortality rate in this study is high.