Dihydrogenistein-Producing Bacterium TM-40 Isolated from Human Feces
Dihydrogenistein-Producing Bacterium TM-40 Isolated from Human Feces
复制标题
从人类粪便中分离出产生二氢金雀异黄酮的细菌 TM-40
DOI：
10.3136/fstr.13.129
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
T. Tsushida
中科院分区：
文献类型：
--
作者：
M. Tamura;M. Ohnishi;H. Nakagawa;T. Tsushida
最近，人们对异黄酮的生物学效应产生了极大的兴趣。肠道植物群在甾体酮的代谢和生物利用度中起重要作用。为了评估饮食中胆甾酮对生理功能的影响，评估肠道植物群对胆甾酮代谢和吸收的作用也很重要。然而，到目前为止，很少有肠道细菌代谢染料木黄酮为二氢异黄酮的报道。以前，我们从一个健康男孩的粪便中分离出了产生二氢大豆苷元的肠道细菌TM-40。菌株TM-40由大豆苷元和大豆苷元产生二氢大豆苷元。TM-40菌株的16 SrRNA部分序列与链形粪杆菌（Coprobacillus catenaformis，Accession no：AB 030218）的16 SrRNA部分序列相似性为93%。该菌株可能是一个新的菌株，并被发现代谢染料木黄酮为二氢异黄酮。我们证明了一个健康男孩的粪便中含有产二氢司坦的肠道细菌。这是第一次报道一个健康男孩的粪便中含有一种产生二氢组氨酸的肠道细菌。
Recently there has been great interest in the biological effects of isoflavones. Intestinal flora play an important role in the metabolism and bioavailability of isoflavones. To estimate the effects of dietary isoflavones on physiological function, it is also important to estimate the role of intestinal flora on the metabolism and absorption of isoflavones. However, to date there have been few reports of intestinal bacteria that metabolize genistein into dihydrogenistein. Formerly, we isolated the dihydrodaidzein-producing intestinal bacterium TM-40 from a healthy boy's feces. The strain TM-40 produce dihydrodaidzein from daidzein and daidzin. The 16S rRNA partial sequence of strain TM-40 showed a 93% similarity to that of Coprobacillus catenaformis (Accession no: AB030218). This strain seems to be a new species and was found to metabolize genistein into dihydrogenistein. We demonstrate that the dihydrogenistein-producing intestinal bacterium was harbored in a healthy boy's feces. This is the first report of a dihydrogenistein-producing intestinal bacterium being harbored in a healthy boy's feces.