Effects of mitomycin C and colchicine on toxin production and cell cycle regulation in the dinoflagellate Alexandrium tamarense
Effects of mitomycin C and colchicine on toxin production and cell cycle regulation in the dinoflagellate Alexandrium tamarense
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DOI：
10.1016/j.hal.2010.10.003
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发表时间：
2011-03-01
期刊：
影响因子：
6.6
通讯作者：
Oshima, Yasukatsu
中科院分区：
文献类型：
--
作者：
Cho, Yuko;Ogawa, Motoo;Oshima, Yasukatsu
研究了两种不同作用机制的代谢抑制剂（丝裂霉素C和秋水仙碱）对塔玛亚历山大藻（Alexandiumtamarense）麻痹性贝类毒素（PST）产生和细胞增殖的影响。用2 μ M丝裂霉素C处理的细胞中的细胞内PST水平逐渐增加，达到最大值176 fmol/细胞（增加6倍）。高浓度的秋水仙素使A. tamarense细胞，即使秋水仙素逮捕其他几种真核细胞类型在M期。在秋水仙素作用下，G（1）期延长的细胞明显不能产生PST。去除秋水仙碱后，细胞增殖和毒素产生恢复;相反，丝裂霉素C的作用是不可逆的。虽然在细胞周期内毒素产生的时间还没有最终确定，A。处于S期的tamarense细胞能够产生PST。(C)2010 Elsevier B. V.保留所有权利。
Paralytic shellfish toxin (PST) production and cell proliferation in the dinoflagellate Alexandrium tamarense were investigated under the influence of two metabolic inhibitors (mitomycin C and colchicine) that have different mechanisms of action. Intracellular PST levels in cells treated with 2 mu M mitomycin C increased gradually, reaching a maximum of 176 fmol/cell (a 6-fold increase). High concentrations of colchicine prolonged G(1) phase in A. tamarense cells, even though colchicine arrests several other eukaryotic cell types in M phase. The cells in prolonged G(1) phase under the influence of colchicine were apparently unable to produce PST. Cell proliferation and toxin production recovered after removal of colchicine; in contrast, the effect of mitomycin C was irreversible. While the timing of toxin production within the cell cycle was not conclusively determined, A. tamarense cells in S phase were able to produce PST. (C) 2010 Elsevier B.V. All rights reserved.