RAPID IMMUNOPEROXIDASE STAINING OF LYMPHOCYTE ANTIGENS USING MICROWAVE IRRADIATION
RAPID IMMUNOPEROXIDASE STAINING OF LYMPHOCYTE ANTIGENS USING MICROWAVE IRRADIATION
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DOI：
10.1002/path.1711480209
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发表时间：
1986-02-01
影响因子：
7.3
通讯作者：
MILIOS, J
中科院分区：
文献类型：
--
作者：
LEONG, ASY;MILIOS, J
介绍了微波辐照在免疫过氧化物酶染色多种不稳定淋巴细胞抗原中的创新应用。320瓦辐照15秒后，冷冻切片极好地粘附在玻片上，表面抗原染色无损。这个简单的过程消除了长时间冷冻干燥或在4°C下干燥的需要。微波辐照也被用来加速一抗的培养。320瓦照射30秒产生的特异性抗原染色强度与在室温下更长的孵育时间所获得的效果一样好。细胞形态学保存也很好，背景染色很少。
The innovative application of microwave irradiation in the immunoperoxidase staining of a wide range of labile lymphocyte antigens is described. Fifteen second irradiation at 320 watts produced excellent adherence of the frozen sections to the glass slides without loss of surface antigen staining. This brief procedure eliminated the need for much longer periods of freeze drying or drying at 4°C. Microwave irradiation was also used to accelerate the incubation of the primary antibody. Thirty seconds of irradiation at 320 watts produced specific antigen staining of an intensity which was as good as that obtained with much longer durations of incubations at room temperature. Cytomorphologic preservation was also good and background staining was minimal.