Passive transfer of Theileria equi antibodies to neonate foals of immune tolerant mares.
Passive transfer of Theileria equi antibodies to neonate foals of immune tolerant mares.
复制标题
马泰勒虫抗体被动转移至免疫耐受母马的新生马驹。
DOI：
10.1016/j.vetpar.2007.10.001
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发表时间：
2008
影响因子：
2.6
通讯作者：
S. Dwivedi
中科院分区：
文献类型：
--
作者：
Sanjay Kumar;Rajender Kumar;A. Gupta;S. Dwivedi
马巴贝斯虫病是一种由马伊勒氏杆菌引起的蜱传播的原虫病，在全球分布，给马业造成严重的经济损失。在流行地区饲养的马科动物通常在幼年时感染，并在其一生中变得免疫耐受。我们研究了从预先免疫的母马出生的新生马驹的被动转移抗体水平。选择潜伏期马绦虫感染的预免疫小马和驴母（各3匹），采用Dot-ELISA法对顺序采集的马驹及其母马血清进行监测，直至产后90天（DPF）。在预免疫的小马和驴母中观察到非常高的抗体滴度。小马驹和驴马驹在2-16和2-10 DPF时抗体最大值分别为1:60 ~ 1:80，之后在63-77和56-63 DPF时抗体最大值降至1:20以下。同时，采用PCR法对血液样本进行检测，监测新生马驹及其坝体的寄生虫携带状况。我们可以证明在大坝的血液样本中有PCR扩增，而在新生儿的血液样本中没有扩增记录。该研究表明，新生马驹出生naïve，被动转移免疫是短暂的，在63-77 DPF后减弱。
Equine babesiosis, a tick transmitted haemoprotozoan disease caused by Theileria equi is globally distributed and responsible for heavy economic losses to the equine husbandry. Equids reared in endemic areas usually pick up infection at an early age and become immune tolerant throughout their life span. We studied the level of passively transferred antibodies in neonate foals born from pre-immuned mares. Latently T. equi infected pre-immuned pony and donkey mares (three each) were selected and T. equi antibody titres in neonates was monitored till 90 days post foaling (DPF) by applying Dot-ELISA on sequentially collected serum samples from foals and their dams. A very high antibody titre was observed in pre-immuned pony and donkey mares. The maximum antibody of 1:60 to 1:80 was observed in pony's and donkey's foal on 2–16 and 2–10 DPF, respectively and thereafter it declined to less than 1:20 on 63–77 and 56–63 DPF. Simultaneously parasite carrying status in neonate foals and their dam was also monitored by applying PCR on blood samples. We could demonstrate PCR amplification in dam's blood samples while no amplification was recorded in neonate's blood samples. This study indicated that new-born foals were born naïve and passively transferred immunity was transitory which wanes after 63–77 DPF.