Tandem reflectron time-of-flight mass spectrometer utilizing photodissociation.
Tandem reflectron time-of-flight mass spectrometer utilizing photodissociation.
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DOI：
10.1021/ac00117a021
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发表时间：
1995-11
影响因子：
7.4
通讯作者：
D. J. Beussman;P. Vlasak;R. D. McLane;M. Seeterlin;C. Enke
中科院分区：
文献类型：
--
作者：
D. J. Beussman;P. Vlasak;R. D. McLane;M. Seeterlin;C. Enke
设计了一种串联飞行时间（TOF）质谱仪，用于获取从气相色谱仪流出的化合物的完整MS/MS光谱。这种应用需要高光谱产生速率、对前体选择和产物光谱都具有单位质量分辨率以及高效的离子利用率。通过在两个阶段都采用反射式飞行时间质量分离以及将激光光诱导解离作为离子裂解方法来实现这些目标。激光脉冲相对于离子提取的精确时间控制使得单一质荷比（m/z）值直至m/z 1000的离子能够被光解，而相邻m/z值的离子基本不受影响。离子包和激光脉冲的会聚焦点使得离子裂解效率高达79%。一个离子门可防止未被选择的前体离子使产物光谱复杂化。可以在最大激光重复频率（目前为200Hz）下产生产物光谱。为了同时对所有产物m/z值实现单位质量分辨率，为第二个飞行时间阶段设计了一种新型反射器。
A tandem time-of-flight (TOF) mass spectrometer has been designed to obtain complete MS/MS spectra from compounds eluting from a gas chromatograph. This application requires high spectral generation rate, unit mass resolution for both precursor selection and product spectra, and efficient ion utilization. These objectives are achieved by reflectron TOF mass separation in both stages and laser photoinduced dissociation as the ion fragmentation method. Careful timing of the laser pulse relative to ion extraction allows ions of a single m/z value up to m/z 1000 to be photodissociated while ions with adjacent m/z values are essentially unaffected. The convergent foci of the ion packet and laser pulse results in ion fragmentation efficiencies as high as 79%. An ion gate prevents the nonselected precursor ions from convoluting the product spectra. Product spectra can be generated at the maximum laser repetition rate (currently 200 Hz). To achieve unit mass resolution for all product m/z values simultaneously, a novel reflectron was designed for the second TOF stage.