Bacillus marmarensis sp nov., an alkaliphilic, protease-producing bacterium isolated from mushroom compost
Bacillus marmarensis sp nov., an alkaliphilic, protease-producing bacterium isolated from mushroom compost
复制标题
DOI：
10.1099/ijs.0.012369-0
复制
发表时间：
2010-07-01
影响因子：
2.8
通讯作者：
Erarslan, Altan
中科院分区：
文献类型：
--
作者：
Denizci, Aziz Akin;Kazan, Dilek;Erarslan, Altan
从位于土耳其马尔马拉地区的Yalova的蘑菇堆肥中分离出一种革兰氏染色阳性的专性嗜碱细菌，命名为菌株GMBE 72（T）。细胞为需氧直杆状，形成近端至端部椭圆形内生孢子。该分离物是过氧化氢酶阳性，氧化酶阴性和能动的，并含有基于内消旋二氨基庚二酸的A1型γ肽聚糖。该菌株在pH 8.0 - 12.5下生长。主要的细胞脂肪酸是anteiso-C-15：0。基因组DNA G + C含量为40.2mol%。基于16S rRNA基因序列分析的系统发育分析表明，菌株GMBE 72（T）属于芽孢杆菌属，与Bacillus pseudofirmus DSM 8715（T）具有98.2%的序列相似性。GMBE 72（T）和B之间的DNA-DNA再结合率为56%。pseudofirmus DSM 8715（T）.根据我们的多相特征，菌株GMBE 72（T）代表芽孢杆菌属的一个新种，建议将其命名为Bacillus marmarensis sp. nov.。模式菌株为GMBE 72（T）（= DSM 21297（T）= JCM 15719（T））。
A Gram-stain-positive, obligately alkaliphilic bacterium designated strain GMBE 72(T) was isolated from mushroom compost from Yalova, located in the Marmara region of Turkey. Cells were aerobic, straight rods and they formed subterminal to terminal ellipsoidal endospores. The isolate was catalase-positive, oxidase-negative and motile and contained a type A1 gamma peptidoglycan based on meso-diaminopimelic acid. The strain grew at pH 8.0-12.5. The major cellular fatty acid was anteiso-C-15:0. The genomic DNA G+C content was 40.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequencing showed that strain GMBE 72(T) belonged to the genus Bacillus and exhibited 98.2% sequence similarity to Bacillus pseudofirmus DSM 8715(T). DNA-DNA reassociation was 56% between GMBE 72(T) and B. pseudofirmus DSM 8715(T). According to our polyphasic characterization, strain GMBE 72(T) represents a novel species of the genus Bacillus, for which the name Bacillus marmarensis sp. nov. is proposed. The type strain is GMBE 72(T) (=DSM 21297(T) =JCM 15719(T)).