Molecular Systems Biology Peer Review Process File toward an Understanding of the Protein Interaction Network of Human Liver Transaction Report-猫眼课题宝

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Jian Wang;K. Huo;Lixin Ma;Liujun Tang;Dong Li;Xiaobi Huang;Yanzhi Yuan;Chunhua Li;Wei Wang-Wei-Wan

(Note注：除了更正可能引起歧义的排印或拼写错误外，信函和报告均未经编辑。信件和裁判报告的原始格式可能不会反映在本汇编中。再次感谢您将您的工作提交给分子系统生物学。我们已经收到了同意评估你的手稿的三位审稿人的回复。正如您将从下面的报告中看到的那样，推荐人发现您的研究主题具有潜在的兴趣。然而，这些问题引起了严重的关切，我恐怕要说，这些关切使报告无法以目前的形式发表。总的来说，评审人员认识到这项工作提供了一个与人类肝脏中表达的蛋白质之间相互作用相关的大型且潜在有用的数据集。尽管如此，他们提出了一系列问题，他们认为，在这项工作适合出版之前，需要令人信服地解决这些问题。我在这里强调两个特别重要的问题：1。评审员对酵母双杂交筛选的假阳性和假阴性评估以及co-IP和GST亲和力实验中使用的对照存在一些重要的技术问题。特别是，请参阅评论家#3提出的观点。似乎需要进一步澄清，在某些情况下需要额外的实验对照。2.评审员认为诱饵蛋白的最初选择没有得到充分的描述，他们担心这组靶向肝脏表达蛋白引入的偏倚需要

(Note: With the exception of the correction of typographical or spelling errors that could be a source of ambiguity, letters and reports are not edited. The original formatting of letters and referee reports may not be reflected in this compilation.) Thank you again for submitting your work to Molecular Systems Biology. We have now heard back from the three referees who agreed to evaluate your manuscript. As you will see from the reports below, the referees find the topic of your study of potential interest. They, however, raise substantial concerns, which, I am afraid to say, preclude its publication in its present form. In general, the reviewers recognized that this work provides a large and potentially useful dataset relevant to interactions among proteins expressed in the human liver. Nonetheless, they raise a series of issues which they felt would need to be addressed convincingly before this work would be appropriate for publications. I highlight here two issues of particular importance: 1. The reviewers had some important technical concerns regarding the assessment of false-positives and false-negatives from the yeast 2-hybrid screen, as well as concerns regarding the controls used in the co-IP and GST-affinity experiments. See, in particular, the points raised by reviewer #3. Additional clarification, and in some cases additional experimental controls, appear needed. 2. The reviewers felt that the initial selection of the bait proteins had not been sufficiently described, and they worried that the bias introduced by this targeted set of liver-expressed proteins needed to

Molecular Systems Biology Peer Review Process File toward an Understanding of the Protein Interaction Network of Human Liver Transaction Report

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