Expression of interleukin 11 and its receptor and their prognostic value in human breast cancer
Expression of interleukin 11 and its receptor and their prognostic value in human breast cancer
复制标题
DOI：
10.1245/aso.2006.05.028
复制
发表时间：
2006-06-01
影响因子：
3.7
通讯作者：
Jiang, Wen G.
中科院分区：
文献类型：
--
作者：
Hanavadi, Satheesha;Martin, Tracey A.;Jiang, Wen G.
背景：最近的实验证据表明白细胞介素 (IL)-11 及其受体在乳腺癌的发生和进展中具有潜在作用。然而，几乎没有临床信息支持这一假设。我们检测了原发性乳腺癌组织样本中 IL-11 及其受体的表达，并将其表达水平与临床结果相关联。 方法：对原发性乳腺癌样本 (n = 109) 和从队列中患者获得的匹配背景组织 (n = 33) 进行冰冻切片和 RNA 提取。来自匹配组织的冰冻切片用 IL-11 和 IL-11 受体抗体进行免疫染色。通过计算机图像分析来分析染色强度。在通过定量聚合酶链式反应进行分析之前，对 RNA 进行逆转录和定量。结果表示为转录本的数量（通过β-肌动蛋白标准化）。将数据与疾病的临床结果进行比较。结果：肿瘤样本中 IL-11 和 IL-11 受体的染色强度明显较高 (P < .01)。与淋巴结阴性样本相比，淋巴结阳性肿瘤样本中 IL-11 的转录水平显着较高 (P = .02)。预后指数较差且组织学分级较差的肿瘤显示出较高水平的IL-11。根据 Kaplan-Meier 生存分析，较高水平的 IL-11 与较差的生存率相关。结论：IL-11 可以作为人类乳腺癌不良预后的预测因子。
Background: Recent experimental evidence has shown a potential role of interleukin (IL)-11 and its receptor in breast cancer development and progression. However, there is little clinical information to support this hypothesis. We examined the expression of IL-11 and its receptor in primary breast cancer tissue samples and correlated their level of expression with the clinical outcome.Methods: Primary breast cancer samples (n = 109) and matched background tissue obtained from patients in the cohort (n = 33) were processed for frozen section and RNA extraction. Frozen sections from matched tissues were immunostained with IL-11 and IL-11 receptor antibodies. Staining intensity was analyzed by computer image analysis. RNA was reverse-transcribed and quantified before analysis by quantitative polymerase chain reaction. Results were expressed as the number of transcripts (standardized by beta-actin). The data were compared with the clinical outcome of the disease.Results: The intensity of staining for both IL-11 and the IL-11 receptor was distinctly high in tumor samples (P < .01). The transcript level of IL-11 was significantly higher in node-positive tumor samples compared with node-negative samples (P = .02). Tumors with a poor prognostic index and poor histological grade showed a higher level of IL-11. A higher level of IL-11 was linked to poorer survival with Kaplan-Meier survival analysis.Conclusions: IL-11 can be a predictor of poor prognosis in human breast cancer.