The nuclear hormone receptor E75A regulates vitellogenin gene (Al‐Vg) expression in the mirid bug Apolygus lucorum
The nuclear hormone receptor E75A regulates vitellogenin gene (Al‐Vg) expression in the mirid bug Apolygus lucorum
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DOI：
10.1111/imb.12365
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发表时间：
2018-04
影响因子：
2.6
通讯作者：
Y-A Tan;X-D Zhao;Y. Sun;D. Hao;J. Zhao;Y.-P. Jiang;L. Bai;L-B Xiao
中科院分区：
文献类型：
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作者：
Y-A Tan;X-D Zhao;Y. Sun;D. Hao;J. Zhao;Y.-P. Jiang;L. Bai;L-B Xiao
绿盲蝽是我国Bt棉的优势害虫。20-羟基蜕皮激素（20 E）在这种昆虫的繁殖中起着关键作用。为了更好地了解20 E调节生殖的机制，核激素受体E75亚型A的Ap。lucorum（A1-E75 A），并分析其表达。Al-E75 A cDNA的2241-bp序列编码预测分子量为69.04 kDa的多肽的开放阅读框。Al-E75 A mRNA在Ap的雌性成虫阶段被检测到。在7日龄的动物中具有峰值表达。Al-E75 A也在几种组织中表达，特别是在脂肪体和卵巢中。通过北方印迹在所有组织中检测到3.2 kb的Al-E75 A mRNA。在用Al-E75 A小干扰RNA处理的雌性成虫中，繁殖力和寿命显著降低。与未处理对照组相比，RNA干扰处理组动物的卵孵化率显著降低。为了研究上述作用的分子机制，选择卵黄蛋白原（A1-Vg）进行进一步研究。Al-Vg的表达模式与Al-E75 A相似，并被20 E上调。敲低Al-E75 A后，Al-Vg的表达谱和蛋白水平下调。这些发现表明，Al-E75 A在Ap中Al-Vg表达的调节中起着至关重要的作用。Lucorum。
Apolygus lucorum is the predominant pest of Bacillus thuringiensis (Bt) cotton in China. 20‐hydroxyecdysone (20E) plays a key role in the reproduction of this insect. To better understand the mechanism underlying 20E‐regulated reproduction, the nuclear hormone receptor E75 isoform‐A of Ap. lucorum (Al‐E75A) was cloned and its expression analysed. A 2241‐bp sequence of Al‐E75A cDNA encoded an open reading frame of a polypeptide with a predicted molecular mass of 69.04 kDa. Al‐E75A mRNA was detected in female adult stages of Ap. lucorum with peak expression in 7‐day‐old animals. Al‐E75A was also expressed in several tissues, particularly in the fat body and ovary. A 3.2 kb Al‐E75A mRNA was detected in all tissues by Northern blot. The fecundity and longevity were significantly decreased in female adults treated with Al‐E75A small interfering RNA. The rates of egg incubation rates were considerably lower in the RNA interference‐treated animals compared to the untreated controls. In order to investigate the molecular mechanism underlying the effects described above, vitellogenin (Al‐Vg) was selected for further investigation. The expression pattern of Al‐Vg was similar to that of Al‐E75A and was up‐regulated by 20E. After knockdown of Al‐E75A, the expression profile of Al‐Vg and the protein levels were down‐regulated. These findings suggest that Al‐E75A plays a crucial role in the regulation of Al‐Vg expression in Ap. lucorum.