Comparison of cation-adjusted Muller-Hinton broth with Iso-Sensitest broth for the NCCLS broth microdilution method
Comparison of cation-adjusted Muller-Hinton broth with Iso-Sensitest broth for the NCCLS broth microdilution method
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DOI：
10.1093/jac/46.3.369
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发表时间：
2000-09-01
影响因子：
5.2
通讯作者：
Poupard, JA
中科院分区：
文献类型：
--
作者：
Koeth, LM;King, A;Poupard, JA
由于方法不同，目前很难比较世界不同地区获得的MIC结果。在本研究中，将nccls推荐的阳离子调整穆勒-辛顿肉汤与欧洲广泛使用的Iso-Sensitest肉汤进行比较。采用NCCLS程序，对124株来自CDC参考集的革兰氏阳性（葡萄球菌和肠球菌）和革兰氏阴性（肠杆菌科和铜绿假单胞菌）分离株进行微肉汤稀释试验，唯一的变量是所使用的培养基。检测12种抗菌药物：阿莫西林-克拉维酸、氨苄西林、环丙沙星、红霉素、庆大霉素、亚胺培南、左氧氟沙星、恶西林、吉氟沙星、甲氧苄啶-磺胺甲恶唑、四环素和万古霉素。万古霉素、红霉素和恶西林仅对革兰氏阳性菌进行评价。甲氧苄啶-磺胺甲恶唑仅对革兰氏阴性菌的一部分进行了评估，因为结果不合比例。124株分离菌分别在美国和英国的一个实验室用两批阳离子调整穆勒-辛顿肉汤和两批iso - sensiest肉汤进行了检测。使用2(4)完全指定的因子分析对数据进行统计评估，以确定由于革兰氏反应、检测地点以及肉汤的类型和/或批次，结果是否存在显著差异。此外，每个肉汤中每种抗菌剂的累积结果与所测MIC稀释度的范围相对应。环丙沙星、左氧氟沙星、吉氟沙星、庆大霉素和四环素的mic用iso - sensiest肉汤比用Mueller-Hinton肉汤略高（稀释倍数为原来的一半）。其他抗菌药物的MIC结果相同。所有代理商的基本协议率和类别协议率具有可比性（分别为88.4-100%和88.2-99.0%）。
Comparison of MIC results obtained in different parts of the world is currently difficult because of variations in methods. In this study, cation-adjusted Mueller-Hinton broth, the NCCLS-recommended medium, was compared with Iso-Sensitest broth, which is widely used in Europe. Microbroth dilution testing, using the NCCLS procedure, was performed on 124 Gram-positive (staphylococci and enterococci) and Gram-negative (Enterobacteriaceae and Pseudomonas aeruginosa) isolates from the CDC reference set, with the only variable being the medium used. Twelve antimicrobial agents were tested: amoxycillin-clavulanic acid, ampicillin, ciprofloxacin, erythromycin, gentamicin, imipenem, levofloxacin, oxacillin, gemifloxacin, trimethoprim-sulphamethoxazole, tetracycline and vancomycin. Vancomycin, erythromycin and oxacillin were only evaluated for the Gram-positive organisms. Trimethoprim-sulphamethoxazole was only evaluated for a subset of Gram-negative organisms because of off-scale results. The 124 isolates were tested in one American and one UK laboratory with two batches of cation-adjusted Mueller-Hinton broth and two of Iso-Sensitest broth. A statistical evaluation of the data used a 2(4) fully specified factorial analysis to determine if there were significant differences in results owing to Gram reaction, site of testing and type and/or batch of broth. In addition, the cumulative results for each antimicrobial agent in each broth were plotted against the range of MIC dilutions tested. MICs of ciprofloxacin, levofloxacin, gemifloxacin, gentamicin and tetracycline were slightly higher (half a doubling dilution) with Iso-Sensitest broth than with Mueller-Hinton broth. MIC results for the other antimicrobial agents were equivalent. Essential and category agreement rates were comparable for all agents (88.4-100% and 88.2-99.0%, respectively).