Expression and intracellular localization of protein phosphatases 2A and 2B, protein kinase a, A-Kinase anchoring protein (AKAP79), and binding of the regulatory (RII) subunit of protein kinase a to AKAP79 in human myometrium.
Expression and intracellular localization of protein phosphatases 2A and 2B, protein kinase a, A-Kinase anchoring protein (AKAP79), and binding of the regulatory (RII) subunit of protein kinase a to AKAP79 in human myometrium.
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人子宫肌层中蛋白磷酸酶 2A 和 2B、蛋白激酶 a、A-激酶锚定蛋白 (AKAP79) 的表达和细胞内定位，以及蛋白激酶 a 的调节 (RII) 亚基与 AKAP79 的结合。
DOI：
10.1016/s1071-5576(03)00136-9
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Smith,GaryD
中科院分区：
文献类型：
--
作者：
Ayres,AllenW;Carr,DanielW;McConnell,DanielS;Lieberman,RichardW;Smith,GaryD
目的：探讨蛋白磷酸酶2A（PP 2A）和2B（PP 2B）、蛋白激酶A（PKA）、A激酶锚定蛋白（AKAP 79）在绝经后和妊娠期子宫肌层中的表达及细胞内定位，以及与AKAP 79结合的PKA（RII亚基）的表达，并将其表达与血中雌二醇、孕酮和催产素水平进行相关性研究。进行了Western免疫印迹、免疫组织化学和RII重叠测定。结果：PP 2A、PKA、AKAP 79及PKA（RII）与AKAP 79结合的表达在三组间无显著差异。与第1组和第3组相比，未分娩组（第2组）中PP 2B的表达显著增加。蛋白磷酸酶2B、PKA和AKAP 79的表达定位于子宫肌层细胞质，而PP 2A定位于血管内皮。结论：人绝经后和妊娠（非临产和临产）子宫肌层均表达PP 2A、PP 2B、PKA、AKAP 79和PKA（RII）-AKAP 79结合蛋白。PP 2A、PKA和AKAP 79表达水平似乎不是分娩时人子宫肌层收缩性的决定因素。PP 2B的表达可能在子宫静止中起作用。未发现蛋白表达与激素水平之间存在相关性。
Objective:To determine the expression and intracellular localization of protein phosphatases 2A (PP2A) and 2B (PP2B), protein kinase A (PKA), and A-kinaes anchoring protein (AKAP79), and expression of PKA (RII subunit) binding to AKAP79 in human postmenopausal and pregnant myometrium and to correlate their expressions to blood levels of estradiol, progesterone, and oxytocin.Methods:Myometrial samples were taken from postmenopausal hysterectomy specimens (group 1, n =5), from pregnant nonlaboring women (group 2, n =7) and pregnant laboring women (group 3, n =5) at cesarean. Western immunoblotting, immunohistochemical, and RII overlay assays were performed. Blood samples were assayed for estradiol, progesterone, and oxytocin levels.Results:There were no significant differences in expression of PP2A, PKA, AKAP79, or PKA(RII) binding to AKAP79 between the three groups. Expression of PP2B was significantly greater in the nonlabor group (group 2) compared with groups 1 and 3. Protein phosphatase 2B, PKA, and AKAP79 expressions were localized in myometrial cytoplasm, but PP2A was localized in blood vessel endothelium. There was no significant correlation between the protein expression and the hormone level in the three groups.Conclusion:Human postmenopausal and pregnant (nonlabor and labor) myometrium expressed PP2A, PP2B, PKA, AKAP79, and PKA (RII)-AKAP79 binding. Levels of PP2A, PKA, and AKAP79 expression did not appear to be determinant of human myometrial contracitility at parturition. Expression of PP2B may play a role in uterine quiescence. No association was found between protein expression and hormone level.