Growth of Particle‐Bearing and Particle‐Free Paramecium aurelia in Axenic Culture
Growth of Particle‐Bearing and Particle‐Free Paramecium aurelia in Axenic Culture
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带颗粒和无颗粒草履虫在无菌培养中的生长
DOI：
10.1111/j.1550-7408.1966.tb01948.x
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发表时间：
1966
影响因子：
2.2
通讯作者：
W. J. van Wagtendonk
中科院分区：
文献类型：
--
作者：
A. Soldo;G. A. Godoy;W. J. van Wagtendonk
剧情简介。在由蛋白酶蛋白胨、胰蛋白酶、酵母核酸、MgSO4.7H2O、TEM-4T（牛脂单甘油酯二乙酰酒石酸酯）、豆甾醇和维生素混合物组成的无菌培养基中培养了几株有颗粒和无颗粒的草草虫。“酵母部分”是以前用于培养这些纤毛虫的培养基中不可或缺的组成部分，现在已被胰酶、酵母核酸和TEM-4T的混合物所取代。
SYNOPSIS. Several strains of particle-bearing and particle-free Paramecium aurelia have been cultivated in an axenic medium composed of proteose peptone, trypticase, yeast nucleic acid, MgSO4.7H2O, TEM-4T (diacetyl tartaric acid esters of tallow monoglycerides), stigmasterol and a mixture of vitamins. The “yeast fraction,” an indispensable component of previous media used for the cultivation of these ciliates has been replaced by a mixture of trypticase, yeast nucleic acid and TEM-4T.
Particle-bearing animals of stock 299 lambda, 138 mu, and 139 pi maintain their particles when cultivated in the medium, whereas particle-bearing animals of stock 51 kappa, 225 kappa and 114 signia do not. With the exception of stock 92 (syngen 3) the medium appears to be selective in its ability to support the growth of animals of the even- but not odd-numbered syngens of P. aurelia.
Maintenance of the particles was dependent only to a small degree upon environmental conditions brought about by changes in pH and temperature. Division of the particles was found to be comparable with the division of the protozoan. Methods for the growth, maintenance and mass cultivation of particle-bearing P. aurelia are given in detail.