Biological and molecular analyses of the acibenzolar-S-methyl-induced systemic acquired resistance in flue-cured tobacco against tomato spotted wilt virus
Biological and molecular analyses of the acibenzolar-S-methyl-induced systemic acquired resistance in flue-cured tobacco against tomato spotted wilt virus
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DOI：
10.1094/phyto-98-2-0196
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发表时间：
2008-02-01
期刊：
影响因子：
3.2
通讯作者：
Pappu, H. R.
中科院分区：
文献类型：
--
作者：
Mandal, B.;Mandal, S.;Pappu, H. R.
番茄斑萎病毒（Tomato spotted wilt virus，TSWV）是烤烟上的一种重要病毒。在温室条件下，通过挑战接种TSWV ASM强毒分离物，研究了酸式苯甲基（ASM）对烤烟系统获得性抗性（SAR）的激活作用，限制了病毒的复制和移动，从而减少了系统感染。在ASM处理后2天内观察到抗性的激活，并且在5天后观察到高水平的抗性。发病机制相关（PR）蛋白基因，PR-3，和不同类别的PR蛋白，如PR-1，PR-3和PR-5的表达检测在ASM治疗后2天，这与TSWV引起的局部病变的数量减少呈负相关。用增加量的ASM（0.25、0.5、1.0、2.0和4.0 g a.i./ 7，000株植物）显示SAR水平增加，如TSWV引起的局部和全身感染的减少所示。抗性水平最高的是在4g活性成分时，但ASM的这种比率也引起植物毒性，导致植物暂时性的叶面斑点和发育不良。随着ASM浓度的增加，TSWV降低与植物毒性之间呈负相关。ASM以1至2 g a.i./ 7，000株植物激活了高水平的抗性，并将植物毒性降至最低。赤霉酸与ASM结合使用可减少ASM引起的发育不良。本研究结果与以往的田间试验表明，ASM是一个潜在的选择烤烟TSWV的管理。
Tomato spotted wilt virus (TSWV) is an economically important virus of flue-cured tobacco. Activation of systemic acquired resistance (SAR) by acibenzolar-S-methyl (ASM) in flue-cured tobacco was studied under greenhouse conditions by challenge inoculation with a severe isolate of TSWV ASM restricted virus replication and movement, and as a result reduced systemic infection. Activation of resistance was observed within 2 days after treatment with ASM and a high level of resistance was observed at 5 days onward. Expression of the pathogenesis-related (PR) protein gene, PR-3, and different classes of PR proteins such as PR-1, PR-3, and PR-5 were detected at 2 days post-ASM treatment which inversely correlated with the reduction in the number of local lesions caused by TSWV. Tobacco plants treated with increased quantities of ASM (0.25, 0.5, 1.0, 2.0, and 4.0 g a.i./7,000 plants) showed increased levels of SAR as indicated by the reduction of both local and systemic infections by TSWV. The highest level of resistance was at 4 g a.i., but this rate of ASM also caused phytotoxicity resulting in temporary foliar spotting and stunting of plants. An inverse correlation between the TSWV reduction and phytotoxicity was observed with the increase of ASM concentration. ASM at the rate of 1 to 2 g a.i./7,000 plants activated a high level of resistance and minimized the phytotoxicity. Use of gibberellic acid in combination with ASM reduced the stunting caused by ASM. Present findings together with previous field experiments demonstrate that ASM is a potential option for management of TSWV in flue-cured tobacco.