THE DOMINANT NON-GIBBERELLIN-RESPONDING DWARF MUTANT (D8) OF MAIZE ACCUMULATES NATIVE GIBBERELLINS
THE DOMINANT NON-GIBBERELLIN-RESPONDING DWARF MUTANT (D8) OF MAIZE ACCUMULATES NATIVE GIBBERELLINS
复制标题
DOI：
10.1073/pnas.85.23.9031
复制
发表时间：
1988-12-01
影响因子：
11.1
通讯作者：
TAKAHASHI, N
中科院分区：
文献类型：
--
作者：
FUJIOKA, S;YAMANE, H;TAKAHASHI, N
内源赤霉素（GAs）进行了检查，从年轻的营养芽的显性突变体，矮-8，GA-非响应，和正常的玉米; GA 44，GA 17，GA 19，GA 20，GA 29，GA 1，和GA 8，早期-13-羟基化途径的成员，从这两种芽全扫描质谱和Kovats保留指数。此外，我们报告了3-epi-GA 1，GA 3，GA 4，GA 5，GA 7，GA 9，GA 12，GA 15，GA 24，GA 34和GA 53的鉴定，使用相同的标准。使用[1，7，12，18 - 14 C4] GA 53和-GA 44、[17- 2 H2] GA 19和[17- 13 C，3 H2] GA 20、-GA 29、-GA 1、-GA 8和-GA 5作为内标物，通过使用毛细管气相色谱法和选择离子监测测量同位素稀释来确定这些GA的内源性水平。矮秆8号的芽积累了较高水平的GA 20、GA 1和GA8。GA_1的积累似乎与基因剂量有关。由于矮8号含有与正常株相同的GA（包括GA 1和GA 3），遗传控制点可能位于GA 1（和GA 3）之后。因此，Dwarf-8可以是GA受体突变体或控制生物活性GA与受体结合的下游产物的突变体。
The endogenous gibberellins (GAs) were examined from young vegetative shoots of the dominant mutant, Dwarf-8, a GA-nonresponder, and normal maize; GA44, GA17, GA19, GA20, GA29, GA1, and GA8, members of the early-13-hydroxylation pathway, were identified from both kinds of shoots by full-scan mass spectra and Kovats retention indices. In addition, we report the identification of 3-epi-GA1, GA3, GA4, GA5, GA7, GA9, GA12, GA15, GA24, GA34, and GA53 by using the same criteria. [1,7,12,18-14C4]GA53 and -GA44, [17-2H2]GA19, and [17-13C, 3H2]GA20, -GA29, -GA1, -GA8, and -GA5 were used as internal standards to determine the endogenous levels of these GAs by measurement of isotope dilution, using capillary gas chromatography and selected ion monitoring. Shoots of Dwarf-8 accumulate relatively high levels of GA20, GA1, and GA8. The accumulation of GA1 appears to be related to gene dosage. Since Dwarf-8 contains the same pattern of GAs as normal (including GA1 and GA3), the genetic control point probably lies after GA1 (and GA3). Thus Dwarf-8 may be a GA receptor mutant or a mutant that controls a product downstream from the binding of the bioactive GA to a receptor.