Varietal differences and chromosome locations of multiple isoforms of starch branching enzyme in wheat endosperm
Varietal differences and chromosome locations of multiple isoforms of starch branching enzyme in wheat endosperm
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DOI：
10.1016/s0031-9422(96)00823-0
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发表时间：
1997-09-01
期刊：
影响因子：
3.8
通讯作者：
Komae, K
中科院分区：
文献类型：
--
作者：
Nagamine, T;Yoshida, H;Komae, K
小麦胚乳淀粉分支酶经Q-SepharoseFast Flow阴离子交换柱层析分离得到Be I和Be II两种不同的亚型。用天然聚丙烯酰胺凝胶电泳法结合酶活性染色，将Be I和Be II精确地分离成几种亚型，并观察到在开花21d后胚乳中表达的主要Be I亚型的品种差异。通过非整倍体分析确定了控制中国春3种Be I亚型表达的基因的染色体位置，结果表明，Be I-1和Be I-2两种异构体受IDL控制，另一种异构体Be I-4为7BL。(C)1997爱思唯尔科学有限公司。保留所有权利。
Starch branching enzyme isolated from wheat endosperm was fractionated into two distinct isoforms, BE I and BE II, using anion-exchange column chromatography on Q-Sepharose Fast Flow. Both BE I and BE II were separated precisely into several isoforms using native polyacrylamide electrophoresis coupled with enzyme activity staining, and varietal differences in the major BE I isoforms expressed in the endosperm 21 days after flowering were observed. Chromosome locations of genes controlling the expression of the three BE I isoforms of 'Chinese Spring' were determined using aneuploid analysis, which indicated that two isoforms, BE I-1 and BE I-2, are controlled by IDL and the other isoform, BE I-4, is 7BL. (C) 1997 Elsevier Science Ltd. All rights reserved.