Optimum design of amphiphilic polymers bearing hydrophobic groups for both cell surface ligand presentation and intercellular cross-linking
Optimum design of amphiphilic polymers bearing hydrophobic groups for both cell surface ligand presentation and intercellular cross-linking
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DOI：
10.1080/09205063.2015.1007414
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发表时间：
2015-02
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影响因子：
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通讯作者：
Masafumi Takeo;Cuicui Li;Masayoshi Matsuda;Hiroko Nagai;Wataru Hatanaka;Tatsuhiro Yamamoto;A. Kishimura;Takeshi Mori;Y. Katayama
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文献类型：
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作者：
Masafumi Takeo;Cuicui Li;Masayoshi Matsuda;Hiroko Nagai;Wataru Hatanaka;Tatsuhiro Yamamoto;A. Kishimura;Takeshi Mori;Y. Katayama
含有疏水烷基的两亲性聚合物有望用于配体在细胞表面的呈现和细胞间的交联。为了探索每种应用的最佳设计，我们合成了8种不同分子量、烷基长度和烷基修饰度的烷基修饰右旋糖酐。我们发现，残酸酯修饰的聚合物在细胞表面的停留时间比棕榈酸酯修饰的聚合物更长。由于聚合物也被生物素修饰，链霉亲和素可以通过生物素-链霉亲和素识别呈现在细胞表面。链霉亲和素在细胞表面的停留时间在酚酸酯修饰的聚合物中比在棕榈酸酯修饰的聚合物中要长。在细胞间交联方面，棕榈酸酯改性聚合物的交联效率高于酚酸酯改性聚合物。本研究结果将有助于设计用于细胞表面工程的酰基修饰聚合物。
Amphiphilic polymers bearing hydrophobic alkyl groups are expected to be applicable for both ligand presentation on the cell surface and intercellular crosslinking. To explore the optimum design for each application, we synthesized eight different acyl-modified dextrans with varying molecular weight, alkyl length, and alkyl modification degree. We found that the behenate-modified polymers retained on the cell surface longer than the palmitate-modified ones. Since the polymers were also modified with biotin, streptavidin can be presented on the cell surface through biotin-streptavidin recognition. The duration of streptavidin on the cell surface is longer in the behenate-modified polymer than the palmitate-modified one. As for the intercellular crosslinking, the palmitate-modified polymers were more efficient than the behenate-modified polymers. The findings in this research will be helpful to design the acyl-modified polymers for the cell surface engineering.