Effect of a resin-modified calcium silicate cement on inflammatory cell infiltration and reparative dentin formation after pulpotomy in rat molars
Effect of a resin-modified calcium silicate cement on inflammatory cell infiltration and reparative dentin formation after pulpotomy in rat molars
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DOI：
10.1111/aej.12568
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发表时间：
2021-10-02
影响因子：
1.6
通讯作者：
Noiri, Yuichiro
中科院分区：
文献类型：
--
作者：
Edanami, Naoki;Ibn Belal, Razi Saifullah;Noiri, Yuichiro
树脂单体和聚合引发剂已被证明对牙髓细胞具有细胞毒性并扰乱成牙本质细胞分化。本研究旨在比较树脂改性硅酸钙水泥（TheraCal LC; TC）和无树脂硅酸钙水泥（ProRoot MTA; PR）对活髓切断术后牙髓愈合的影响。用PR或TC对8周龄大鼠的上颌第一磨牙进行活髓切断术。在第1、3、7、14和28天后，通过显微计算机断层扫描、苏木精-伊红染色和抗CD 68（泛巨噬细胞标记物）的免疫染色评估牙髓反应。结果表明，与PR相比，TC活髓切断术诱导了包括CD 68阳性巨噬细胞在内的炎性细胞的持续浸润，并延迟了修复性牙本质的形成，尽管两种材料都允许牙髓长期愈合。因此，树脂改性TC是不作为生物相容性，也没有生物诱导无树脂PR时，应用于大鼠磨牙的健康牙髓。
Resin monomers and polymerisation initiators have been shown to be cytotoxic for pulp cells and to disturb odontoblast differentiation. This study aimed to compare the effect of a resin-modified calcium silicate cement (TheraCal LC; TC) and a resin-free calcium silicate cement (ProRoot MTA; PR) on pulpal healing after pulpotomy. Pulpotomy was performed on the maxillary first molars of 8-week-old rats using either PR or TC. After 1, 3, 7, 14 and 28 days, pulpal responses were assessed by micro-computed tomography, haematoxylin-eosin staining and immunostaining against CD68, which is a pan-macrophage marker. The results showed that pulpotomy with TC induced persistent infiltration of inflammatory cells, including CD68-positive macrophages, and delayed the formation of reparative dentin as compared with that with PR, although both materials allowed pulpal healing over the long term. Therefore, resin-modified TC was not as biocompatible nor bioinductive as resin-free PR when applied on the healthy pulp of rat molars.