Phenotype of recovering lymphoid cell populations after marrow transplantation.
Phenotype of recovering lymphoid cell populations after marrow transplantation.
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DOI：
10.1084/jem.161.6.1483
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发表时间：
1985-06-01
影响因子：
15.3
通讯作者：
Smith, B R
中科院分区：
文献类型：
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作者：
Ault, K A;Antin, J H;Ginsburg, D;Orkin, S H;Rappeport, J M;Keohan, M L;Martin, P;Smith, B R
四名接受骨髓移植的患者在移植后期间进行了连续研究，以确定恢复淋巴细胞类型的模式。三名患者接受了T细胞耗尽的、HLA相合的骨髓，一名患者接受了来自同卵双胞胎的未经治疗的骨髓。用25对不同的单抗标记血淋巴细胞。在每个样本中，一个抗体与荧光素偶联，一个抗体与藻红蛋白偶联，从而允许使用荧光激活细胞分类器同时评估这两个标记物的表达。常规使用14种抗体，包括HLE、Leu-M3、Leu-4、Leu-1、Leu-5、Leu-9、Leu-6、Leu-2、Leu-3、HLA-DR、Leu-7、Leu-11、Leu-15和Leu-12。其他抗体被用来进一步定义一些人群。这项研究使我们能够确定六种不同的细胞类型，它们在移植后第90天出现在所有四名患者中，占所有循环淋巴细胞的90%-100%。这些细胞类型是：(A)表达Leu-1、Leu-4、Leu-9、Leu-5、Leu-3和可变数量的HLA-DR的T辅助细胞；(B)表达Leu-1、Leu-4、Leu-9、Leu-5、Leu-2的T抑制细胞，以及可变数量的HLA-DR；(C)表达Leu-12、B1、HLA-DR、IGD和IgM但不表达T细胞抗原的B细胞；(D)异常的B细胞表型(Leu-1B)，表达所有B细胞标记，且有少量Leu-1，但不表达其他T细胞抗原；(E)自然杀伤(NK)细胞，表达Leu-11、Leu-15、Leu-5，但不表达其他T细胞或B细胞标记；(F)NK细胞表达Leu-11、Leu-15、Leu-5，但低水平Leu-2。这两种NK细胞在部分但不是全部细胞上也表达Leu-7。这些细胞类型的相对频率在患者之间和随时间变化，但显著的发现是相对较少的成熟T细胞，大量的NK细胞，以及在所有三名形成B细胞的患者中，异常的Leu-1B细胞比传统的B细胞占优势。分选实验证实了主要NK细胞表型的NK活性，DNA分析证实所有研究的细胞都来自供者来源。此外，对一名患者的免疫球蛋白基因的分析表明，Leu-1B细胞没有克隆性重排。
Four patients who received bone marrow transplants were studied sequentially during the posttransplant period to define the pattern of recovering lymphoid cell types. Three patients received T cell- depleted, HLA-matched marrow, and one received untreated marrow from an identical twin. Blood lymphoid cells were labeled with 25 different pairs of monoclonal antibodies. In each sample, one antibody was conjugated to fluorescein and one to phycoerythrin, thus allowing simultaneous assessment of the expression of the two markers using the fluorescence activated cell sorter. A total of 14 antibodies were used, routinely including HLE, Leu-M3, Leu-4, Leu-1, Leu-5, Leu-9, Leu-6, Leu- 2, Leu-3, HLA-DR, Leu-7, Leu-11, Leu-15, and Leu-12. Other antibodies were used to further define some populations. This study has allowed us to define six distinct cell types that have appeared in all four patients by day 90 posttransplantation, and which account for 90-100% of all circulating lymphoid cells. These cell types are (a) T helper cells expressing Leu-1, Leu-4, Leu-9, Leu-5, Leu-3, and variable amounts of HLA-DR; (b) T suppressor cells expressing Leu-1, Leu-4, Leu- 9, Leu-5, Leu-2, and variable amounts of HLA-DR; (c) B cells expressing Leu-12, B1, HLA-DR, IgD, and IgM, but none of the T cell antigens; (d) an unusual B cell phenotype (Leu-1 B) expressing all of the B cell markers, and also having low amounts of Leu-1, but none of the other T cell antigens; (e) natural killer (NK) cells expressing Leu-11, Leu-15, Leu-5 but none of the other T cell or B cell markers; (f) NK cells expressing Leu-11, Leu-15, Leu-5, and low levels of Leu-2. Both NK types also express Leu-7 on some, but not all cells. The relative frequencies of these cell types varied among the patients and with time, but the striking findings were the presence of relatively few mature T cells, large numbers of NK cells, and the preponderance of the unusual Leu-1 B cell over conventional B cells in all three patients who developed B cells. Sorting experiments confirmed the NK activity of the major NK cell phenotypes, and DNA analysis confirmed that all of the cells studied were of donor origin. In addition, analysis of Ig genes in one patient showed that the Leu-1 B cells were not clonally rearranged.(ABSTRACT TRUNCATED AT 400 WORDS)