A POSTTRANSLATIONAL MODIFICATION OF THE PHOTOSYSTEM-II SUBUNIT CP29 PROTECTS MAIZE FROM COLD STRESS
A POSTTRANSLATIONAL MODIFICATION OF THE PHOTOSYSTEM-II SUBUNIT CP29 PROTECTS MAIZE FROM COLD STRESS
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DOI：
10.1074/jbc.270.15.8474
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发表时间：
1995-04-14
影响因子：
4.8
通讯作者：
BASSI, R
中科院分区：
文献类型：
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作者：
BERGANTINO, E;DAINESE, P;BASSI, R
玉米植株对低温胁迫的抗性与光处理后类囊体膜上出现一种新的叶绿素a/b结合蛋白有关。对该冷诱导蛋白进行了分离、氨基酸序列分析和放射性前体标记，结果表明该蛋白是次要的叶绿素a/b蛋白CP29翻译后磷酸化修饰的产物，而不是冷调控基因或未加工的CP29前体的产物。在这里，我们发现CP29的活性表现出不同于先前所描述的类囊体膜蛋白的独特特征，并且受苯醌位置的氧化还原状态的调节。最后，我们发现，不能进行磷酸化的玉米植株对冷诱导的光抑制的敏感性增强。
The resistance of maize plants to cold stress has been associated with the appearance of a new chlorophyll a/b binding protein in the thylakoid membrane following chilling treatment in the light. The cold-induced protein has been isolated, characterized by amino acid sequencing, and pulse labeled with radioactive precursors, showing that it is the product of post-translational modification by phosphorylation of the minor chlorophyll a/b protein CP29 rather than the product of a cold-regulated gene or an unprocessed CP29 precursor. We show here that the CP29 kinase activity displays unique characteristics differing from previously described thylakoid kinases and is regulated by the redox state of a quinonic site. Finally, we show that maize plants unable to perform phosphorylation have enhanced sensitivity to cold-induced photoinhibition.