Therapeutic effect of Pseudomonas aeruginosa phage YH30 on mink hemorrhagic pneumonia
Therapeutic effect of Pseudomonas aeruginosa phage YH30 on mink hemorrhagic pneumonia
复制标题
铜绿假单胞菌噬菌体YH30对水貂出血性肺炎的治疗作用
DOI：
10.1016/j.vetmic.2016.03.016
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发表时间：
2016-07-15
影响因子：
3.3
通讯作者：
Han, Wenyu
中科院分区：
文献类型：
--
作者：
Gu, Jingmin;Li, Xinwei;Han, Wenyu
由铜绿假单胞菌引起的出血性肺炎仍然是养殖水貂中最昂贵的传染病之一，通常在水貂生产过程中导致巨大的经济损失。本研究的目的是探讨利用噬菌体治疗水貂出血性肺炎的潜力。利用水貂源性铜绿假单胞菌（血清型G） D7菌株作为宿主，分离出一种广泛宿主范围的足病毒科噬菌体YH30。YH30的基因组为72,192 bp (54.92% G + C)，包含86个开放阅读框，缺乏编码已知毒力因子、整合相关蛋白或抗生素耐药性决定因素的区域。这些特性使YH30有资格用于噬菌体治疗。一项治疗实验的结果表明，单次鼻内给药YH30足以治愈水貂出血性肺炎。yh30保护水貂血液和肺中铜绿假单胞菌的平均菌落计数在细菌攻击24 h内小于10(3)CFU/mL (g)，最终无法检测到，而未保护水貂的菌落计数超过10(8)CFU/mL (g)。此外，YH30可显著改善出血性肺炎水貂肺损伤的病理表现。我们的工作证明了噬菌体治疗铜绿假单胞菌引起的水貂出血性肺炎的潜力。(C) 2016 Elsevier B.V.版权所有
Hemorrhagic pneumonia caused by Pseudomonas aeruginosa remains one of the most costly infectious diseases among farmed mink and commonly leads to large economic losses during mink production. The objective of this study was to investigate the potential of using phages as a therapy against hemorrhagic pneumonia in mink. A broad-host-range phage from the Podoviridae family, YH30, was isolated using the mink-originating P. aeruginosa (serotype G) D7 strain as a host. The genome of YH30 was 72,192 bp (54.92% G + C), contained 86 open reading frames and lacked regions encoding known virulence factors, integration-related proteins or antibiotic resistance determinants. These characteristics make YH30 eligible for use in phage therapy. The results of a curative treatment experiment demonstrated that a single intranasal administration of YH30 was sufficient to cure hemorrhagic pneumonia in mink. The mean colony count of P. aeruginosa in the blood and lung of YH30-protected mink was less than 10(3) CFU/mL (g) within 24 h of bacterial challenge and ultimately became undetectable, whereas that in unprotected mink reached more than 10(8) CFU/mL (g). Additionally, YH30 dramatically improved the pathological manifestations of lung injury in mink with hemorrhagic pneumonia. Our work demonstrates the potential of phages to treat P. aeruginosa-caused hemorrhagic pneumonia in mink. (C) 2016 Elsevier B.V. All rights reserved.