The gene for the large subunit of ribulose-1,5-bisphosphate carboxylase in Euglena gracilis chloroplast DNA: location, polarity, cloning, and evidence for an intervening sequence.
The gene for the large subunit of ribulose-1,5-bisphosphate carboxylase in Euglena gracilis chloroplast DNA: location, polarity, cloning, and evidence for an intervening sequence.
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细小裸藻叶绿体 DNA 中核酮糖 1,5-二磷酸羧化酶大亚基的基因：位置、极性、克隆和插入序列的证据。
DOI：
10.1093/nar/10.11.3427
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发表时间：
1982
影响因子：
14.9
通讯作者：
Hallick,RB
中科院分区：
文献类型：
--
作者：
Stiegler,GL;Matthews,HM;Bingham,SE;Hallick,RB
利用玉米和衣藻核酮糖-1，5-二磷酸羧化酶基因的限制性内切酶片段，通过异源杂交，定位了纤细眼虫（Euglena gracilis）叶绿体DNA的核酮糖-1，5-二磷酸羧化酶大亚基（LS）基因。EuglenaLS基因与EuglenaRNA基因具有相同的极性，已被定位在EcoRI片段EcoA内的Pst I、Pvu I和HindIII位点上。Euglenachloroplast DNA与Chlamydomonaschloroplast LS基因的887 bp的内部片段互补的区域被0.5- 1.1kbp的非互补序列中断。这是第一个位于眼虫基因组上的叶绿体蛋白基因，也是第一个在叶绿体蛋白基因中存在插入序列的证据。
The gene for the large subunit (LS) of ribulose-1,5-bisphosphate carboxylase ofEuglena gracilisZ chloroplast DNA has been mapped by heterologous hybridization with DNA restriction fragments containing internal sequences from theZea mayaandChlamydomonas reinhardiiLS genes. TheEuglenaLS gene which has the same polarity as theEuglenarRNA genes has been located with respect toPstI,PvuI, andHindIII sites within theEcoRI fragmentEcoA. The region ofEuglenachloroplast DNA complementary to an 887 bp internal fragment from theChlamydomonaschloroplast LS gene is interrupted by a 0.5–1.1 kbp non-complementary sequence. This is the first chloroplast protein gene located on theEuglenagenome, and the first evidence for an intervening sequence within any chloroplast protein gene.