Chinese Yellow Wine and Red Wine Inhibit Matrix Metalloproteinase-2 and Improve Atherosclerotic Plaque in LDL Receptor Knockout Mice
Chinese Yellow Wine and Red Wine Inhibit Matrix Metalloproteinase-2 and Improve Atherosclerotic Plaque in LDL Receptor Knockout Mice
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DOI：
10.1111/j.1755-5922.2009.00132.x
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发表时间：
2010-01-01
影响因子：
3.1
通讯作者：
Huang, Dilai
中科院分区：
文献类型：
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作者：
Guo, Hangyuan;Liu, Longbin;Huang, Dilai
本研究发现黄酒可抑制培养大鼠血管平滑肌细胞中同型半胱氨酸（HCY）诱导的细胞外基质金属蛋白酶-2 （MMP-2）的产生。中国黄酒与动脉粥样硬化或体内MMP-2的关系尚不清楚。32只低密度脂蛋白受体敲除小鼠在高脂肪和l-蛋氨酸饮食中出现血浆高同型半胱氨酸血症和动脉粥样硬化。随机分为黄酒组（n = 8）、红酒组（n = 8）、乙醇组（n = 8）和对照组（n = 8）， 14周后处死。四组患者血浆总胆固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平差异均无统计学意义。与其他3组相比，黄酒组血浆HCY显著降低（P < 0.01）。与乙醇组和对照组相比，黄酒组和红葡萄酒组明显减少动脉粥样硬化病变面积（P < 0.001）。然而，黄酒组和红酒组之间没有显著差异。与对照组和乙醇组相比，黄酒组主动脉窦中MMP-2的生成降低了26.8%和23.6%，主动脉弓中MMP-2的激活降低了32.6%和27.3%；主动脉窦中MMP-2的生成减少了25.7%和22.4%，主动脉弓中MMP-2的激活减少了30.2%和26.6%。上述结果提示，黄酒和红酒均能抑制MMP-2，改善动脉粥样硬化，可能黄酒和红酒都通过抑制MMP-2对心血管疾病有有益作用。
Our previous study found that Chinese yellow wine could inhibit the production of homocysteine (HCY) induced extracellular matrix metalloproteinase-2 (MMP-2) in the cultured rat vascular smooth muscle cells. Little is known about the relationship between Chinese yellow wine and atherosclerosis or MMP-2 in vivo. Thirty-two LDL Receptor knockout mice on a high-fat and l-methionine diet developed plasma hyperhomocysteinemia and atherosclerosis. They were randomly divided into yellow wine group (n = 8), red wine group (n = 8), ethanol group (n = 8), and control group (n = 8), they were sacrificed after 14 weeks. There were no significant differences with plasma total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels in the four groups. Plasma HCY was significantly decreased in the yellow wine group compared to the other three groups (P < 0.01). Yellow wine and red wine groups significantly reduced the atherosclerosis lesion area compared to ethanol and control groups (P < 0.001). However, there was no significant discrepancy between the yellow wine group and red wine group. Compared to the control group and ethanol group, the production of MMP-2 reduced 26.8% and 23.6% in the aortic sinus and the activation of MMP-2 reduced 32.6% and 27.3% in the aortic arch in the yellow wine group; the production of MMP-2 reduced 25.7% and 22.4% in the aortic sinus and the activation of MMP-2 reduced 30.2% and 26.6% in the aortic arch in the red wine group. These results suggest that Chinese yellow wine and red wine can inhibit MMP-2 and improve atherosclerosis, and maybe both Chinese yellow wine and red wine have beneficial effects on cardiovascular disease by inhibiting MMP-2.