Phosphate transporters from the higher plant Arabidopsis thaliana
Phosphate transporters from the higher plant Arabidopsis thaliana
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DOI：
10.1073/pnas.93.19.10519
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发表时间：
1996-09-17
影响因子：
11.1
通讯作者：
Raghothama, KG
中科院分区：
文献类型：
--
作者：
Muchhal, US;Pardo, JM;Raghothama, KG
从磷饥饿拟南芥根的mRNA文库中分离到两个编码磷酸盐转运蛋白的cDNA（AtPT 1和AtPT 2），它们与酿酒酵母（Saccharomycescerevisiae）、粗糙脉孢菌（Neurosporacrassa）和菌根真菌Glomusversiforme的高亲和力磷酸盐转运蛋白的氨基酸序列有78%的同源性。AtPT 1和AtPT 2多肽是整合的膜蛋白，预测其含有12个跨膜结构域，这些跨膜结构域被大的带电亲水区域分成两组，每组6个。表达后，AtPT 1和AtPT 2都能够补充缺乏高亲和力磷酸盐转运活性的酵母菌株NS 219的pho84突变体表型。AtPT 1和AtPT 2代表两种不同的，A. thaliana.这两个基因的转录本在根中表达，在叶中检测不到。它们的mRNA的稳态水平增加，以响应磷酸盐饥饿。
Two cDNAs (AtPT1 and AtPT2) encoding plant phosphate transporters have been isolated from a library prepared with mRNA extracted from phosphate-starved Arabidopsis thaliana roots, The encoded polypeptides are 78% identical to each other and show high degree of amino acid sequence similarity with high-affinity phosphate transporters of Saccharomyces cerevisiae, Neurospora crassa, and the mycorrhizal fungus Glomus versiforme. The AtPT1 and AtPT2 polypeptides are integral membrane proteins predicted to contain 12 membrane-spanning domains separated into two groups of six by a large charged hydrophilic region, Upon expression, both AtPT1 and AtPT2 were able to complement the pho84 mutant phenotype of yeast strain NS219 lacking the high-affinity phosphate transport activity, AtPT1 and AtPT2 are representatives of two distinct, small gene families in A. thaliana. The transcripts of both genes are expressed in roots and are not detectable in leaves. The steady-state level of their mRNAs increases in response to phosphate starvation.