Expression of lymphotoxin-beta (LT-β) in chronic inflammatory conditions
Expression of lymphotoxin-beta (LT-β) in chronic inflammatory conditions
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DOI：
10.1002/path.1249
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发表时间：
2003-01-01
影响因子：
7.3
通讯作者：
Cohen, J
中科院分区：
文献类型：
--
作者：
Agyekum, S;Church, A;Cohen, J
在基因敲除和转基因小鼠系统中的功能研究已经表明，α-光敏素和β-光敏素（LT-α和LT-β）在外周淋巴器官发育中具有根本重要性，但仍不清楚这些细胞因子在成人免疫应答和疾病发病机制中发挥什么作用。在这项研究中，一个多克隆抗血清的人LT-β的免疫组化研究LT-β在正常和病变组织中的分布。在肠道、淋巴结、脾脏和扁桃体中，各种淋巴细胞类型上存在一些LT-β。相比之下，在受慢性炎性疾病或感染影响的组织（例如炎性肠病）和从结节病和结核病患者获得的淋巴结中，在浆细胞和CD 4 + T细胞亚群上观察到LT-β的强染色。在结核性和结节性淋巴结中，LT-β表达也发生在肉芽肿内的一些但不是所有上皮样组织细胞和多核巨细胞上。这些发现支持LT-β在人类疾病中的作用，并表明它可能代表各种常见感染或炎症性疾病的治疗靶点。版权所有（C）2002约翰威利父子有限公司
Functional studies in gene-knockout and transgenic mice systems have shown that lymphotoxin-alpha and lymphotoxin-beta (LT-alpha and LT-beta) are of fundamental importance in peripheral lymphoid organ development, but it remains unclear what role these cytokines have to play in the adult immune response and in the pathogenesis of disease. In this study, a polyclonal anti-serum to human LT-beta was used to investigate the distribution of LT-beta by immohistochemistry in normal and diseased tissues. In the gut, lymph nodes, spleen, and tonsil, there was some LT-beta present on a variety of lymphoid cell types. In contrast, strong staining for LT-beta was observed on plasma cells and a subpopulation of CD4+ T cells in tissues affected by chronic inflammatory disease or infection, for example in inflammatory bowel disease, and in lymph nodes obtained from patients with sarcoidosis and tuberculosis. In tuberculous and sarcoid lymph nodes, LT-beta expression also occurred on some but not all epithelioid histiocytes within granulomas and on multi-nucleated giant cells. These findings support a role for LT-beta in human disease and suggest that it might represent a therapeutic target in a variety of common infective or inflammatory disorders. Copyright (C) 2002 John Wiley Sons, Ltd.