A two-photon AIEgen for simultaneous dual-color imaging of atherosclerotic plaques
A two-photon AIEgen for simultaneous dual-color imaging of atherosclerotic plaques
复制标题
用于动脉粥样硬化斑块同时双色成像的双光子 AIEgen
DOI：
10.1039/c8mh01293h
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发表时间：
2019
影响因子：
13.3
通讯作者：
Zheng Lei
中科院分区：
文献类型：
--
作者：
Situ Bo;Gao Meng;He Xiaojing;Li Shiwu;He Bairong;Guo Fengxia;Kang Chunmin;Liu Shan;Yang Lei;Jiang Meijuan;Hu Yanwei;Tang Ben Zhong;Zheng Lei
动脉内脂质的高分辨率成像对于获得关于其在动脉粥样硬化中的作用的基础知识具有重要价值，但仍然缺乏理想的探针。在这项研究中，我们报告了一种智能探针，即IND与聚集诱导发射的性质，用于双光子可视化的细胞系和动脉内的脂质。利用高脂质特异性、深层组织穿透性和优异的双光子成像性能，IND能够对培养细胞中的脂质进行高分辨率成像，并原位绘制小鼠动脉粥样硬化斑块中的三维脂质分布，而无需繁琐的组织学制备。重要的是，我们发现IND在分子单体和二聚体状态下具有不同的发射特性;此外，我们发现其在组织微环境中的脂质/水的单激发双色成像以揭示动脉粥样硬化斑块的超微结构方面的显着应用。本文所述的发现提供了一种简单的工具，具有最小的破坏性程序，用于在组织水平上研究动脉粥样硬化，并提供了一种强大的策略，用于开发具有颜色切换能力的基于成像的诊断技术。
High-resolution imaging of lipids within the artery is of great value to obtain fundamental knowledge about their roles in atherosclerosis, but ideal probes are still lacking. In this study, we report a smart probe, namely, IND with aggregation-induced emission property for two-photon visualization of lipids within cell lines and arteries. Taking advantage of high lipid specificity, deep tissue penetrability, and excellent two-photon imaging performance, IND is capable of high-resolution imaging of lipids in cultured cells and in situ mapping of three-dimensional lipid distributions in mouse atherosclerotic plaques without tedious histological preparations. Importantly, we find that IND exhibits distinct emissive properties in the molecular monomer and dimer states; also, we uncover its remarkable applications for single-excitation dual-color imaging of lipid/water in the tissue microenvironment to reveal the ultra-structure of atherosclerotic plaques. The findings described herein provide a simple tool with minimal disruptive procedures for studying atherosclerosis at the tissue level and a powerful strategy for the development of imaging-based diagnostic techniques with color switching capability.