An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants
An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants
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DOI：
10.1007/978-1-60761-702-0_15
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Tang, Guiliang
中科院分区：
文献类型：
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作者：
Jia, Xiaoyun;Mendu, Venugopal;Tang, Guiliang
微RNA（microRNAs，miRNAs）是一类长度约为22个核苷酸的非编码RNA，在植物发育和非生物胁迫中起重要作用。我们已经开发了一个简单但有效的阵列平台，用于分析来自各种植物物种的植物miRNA。该阵列由188个非冗余miRNA探针组成，可以检测大多数植物物种的保守和物种特异性miRNA，包括拟南芥，水稻和白杨。在这一章中，我们描述了一个协议，用于开发的miRNA阵列平台，它可以用来识别不同植物物种的胁迫响应的miRNA。对暴露于不同非生物胁迫条件下的烟草幼苗中的miRNAs的分析表明，许多miRNAs，miR398、miR399、miR408、miR156、miR164和miR168，对胁迫有响应。这一全面且易于遵循的方案将有助于研究miRNAs在植物胁迫反应中的作用。
MicroRNAs (miRNAs) arc approximately 22-nucleotide (nt)-long non-coding RNAs that play a key role in plant development and abiotic stresses. We have developed a simple but effective array platform for profiling plant miRNAs from various plant species. The array is composed of 188 non-redundant miRNA probes that can detect both conserved and species-specific miRNAs from most plant species, including Arabidopsis, rice, and poplar. In this chapter, we describe a protocol for developing the miRNA array platform, which can be used to identify stress-responsive miRNAs in diverse plant species. Profiling of miRNAs in tobacco seedlings exposed to different abiotic stress conditions has revealed that a number of miRNAs, miR398, miR399, miR408, miR156, miR164, and miR168, were responsive to stresses. This comprehensive and easy-to-follow protocol will be useful for studying roles of miRNAs in plant stress response.