Sex vesicle-associated nucleolar organizers in mouse spermatocytes: localization, structure, and function.
Sex vesicle-associated nucleolar organizers in mouse spermatocytes: localization, structure, and function.
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小鼠精母细胞中性囊泡相关核仁组织者：定位、结构和功能。
DOI：
10.1159/000131625
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
A. Stahl
中科院分区：
文献类型：
--
作者：
B. Knibiehler;C. Mirre;M. Hartung;P. Jean;A. Stahl
选择性银染色表明，在小鼠精母细胞发育过程中，常染色体双价体含有转录活性核仁组织者，与性囊相关。在粗线期后期，核仁组织者覆盖了性囊泡中离核膜最远的部分。原位杂交显示在银阳性物质中存在rDNA。核仁由常染色体二价体形成，显示出一个大的纤维中心，周围是一个电子不透明的纤维区。通过原位杂交、NOR银染色和电子显微镜研究了早期、中期和晚期核仁与性囊泡的联系。这些观察结果使我们能够进一步确定核仁组分与X-Y对的关系。
Selective silver staining demonstrated that autosomal bivalents containing transcriptively active nucleolar organizers associated with the sex vesicle during pachytene of mouse spermatocytes. Later in pachytene, the nucleolar organizers covered the portion of the sex vesicle furthest from the attachment to the nuclear envelope. Hybridization in situ revealed the presence of rDNA in the silver-positive material. The nucleolus, formed from an autosomal bivalent, exhibited a large fibrillar center surrounded by an electron-opaque fibrillar zone. The nucleolar association with the sex vesicle was studied at early, middle, and late pachytene by hybridization in situ, NOR silver staining, and electron microscopy. These observations enabled us to further define the relationships of the nucleolar components with the X-Y pair.