Differential binding of Mg2+, Zn2+, and Cd2+ at two sites in a hammerhead ribozyme motif, determined by 15N NMR.
Differential binding of Mg2+, Zn2+, and Cd2+ at two sites in a hammerhead ribozyme motif, determined by 15N NMR.
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通过 15N NMR 测定，Mg2、Zn2 和 Cd2 在锤头核酶基序中两个位点的差异结合。
DOI：
10.1021/ja049804v
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发表时间：
2004
影响因子：
15
通讯作者：
Jones,RogerA
中科院分区：
文献类型：
--
作者：
Wang,Gang;Gaffney,BarbaraL;Jones,RogerA
在已知的金属结合部位G10.1用[8-13C-1，7，NH2-15N3]-鸟苷合成了锤头状核酶结构域II的十聚体双链模型，作为比较，在G16.2位合成了[2-13C-1，7，NH2-15N3]-鸟苷。在添加Mg~(2+)、Cd~(2+)和Zn~(2+)的过程中，标记的N7的15N核磁共振化学位移显示出每种金属在G10.1比G16.2处的结合偏好相同。这些结果表明，15N标记可以用来评价不同金属，包括镁离子与给定的氮的结合，以及比较不同位置的结合潜力。
A decamer duplex model of Domain II of the hammerhead ribozyme was synthesized with [8-13C-1,7,NH2-15N3]-guanosine at the known metal binding site, G10.1 and, for comparison, [2-13C-1,7,NH2-15N3]-guanosine at G16.2. The15N NMR chemical shifts of the labeled N7s monitored during addition of Mg2+, Cd2+, and Zn2+showed the same preference for binding at G10.1 over G16.2 for each metal. These results demonstrate that15N labeling can be used to evaluate the binding of different metals, including Mg2+, to a given nitrogen, as well as to compare the binding potential of different sites.