Synthesis of a peptide that can translocate to the endoplasmic reticulum.
Synthesis of a peptide that can translocate to the endoplasmic reticulum.
复制标题
合成可易位至内质网的肽。
DOI：
10.1016/j.bbrc.2015.03.080
复制
发表时间：
2015
影响因子：
3.1
通讯作者：
and Koichiro Ozawa.
中科院分区：
文献类型：
--
作者：
Kanako Nakatsu;Toru Hosoi;Keisuke Toyoda;and Koichiro Ozawa.
未折叠蛋白在内质网（ER）中的积累导致ER应激，这与疾病的发展有关。在本研究中，我们合成了一种进入细胞并易位到ER的肽。该肽具有异硫氰酸荧光素（FITC）、HIV-TAT、小α A晶状体蛋白和KDEL序列。我们证明了这种肽进入细胞并易位到ER。时程实验表明，该肽在cos-7细胞内质网中存在16 h。此外，我们通过荧光免疫染色和SDS-PAGE检测细胞中的全长肽。该肽还进入神经胶质细胞和神经元细胞。这些结果表明，这种肽具有进入细胞并在ER发挥伴侣活性的能力，并为新药的开发提供了见解。
The accumulation of unfolded proteins in the endoplasmic reticulum (ER) leads to ER stress, which has been implicated in the development of diseases. In the present study, we synthesized a peptide that entered cells and translocated to the ER. This peptide possessed fluorescein isothiocyanate (FITC), HIV-TAT, mini-αA-crystallin, and KDEL sequences. We demonstrated that this peptide entered cells and translocated to the ER. Time course experiments revealed that this peptide existed in the ER of cos-7 cells for 16 h. Furthermore, we detected the full-length peptide in cells by fluorescent immunostaining followed by SDS-PAGE. The peptide also entered glial and neuronal cells. These results suggest that this peptide has the ability to enter cells and exert chaperone activity at the ER, and provide an insight into the development of new drugs.