COORDINATE EXPRESSION OF X-HAPTENS AND Y-HAPTENS DURING MURINE EMBRYOGENESIS
COORDINATE EXPRESSION OF X-HAPTENS AND Y-HAPTENS DURING MURINE EMBRYOGENESIS
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DOI：
10.1016/0012-1606(86)90379-9
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发表时间：
1986-03-01
影响因子：
2.7
通讯作者：
HAKOMORI, S
中科院分区：
文献类型：
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作者：
FENDERSON, BA;HOLMES, EH;HAKOMORI, S
X半抗原(Gal.Beta.fwdarw.4[Fuc.Alpha.1.fwdarw.3]GlcNAc)在压实过程中可能在卵裂球的粘连中起重要作用。因此，我们使用特征明确的单抗对乳糖系列碳水化合物链岩藻糖化的发育变化以及这些岩藻糖化变化的酶基础进行了更深入的研究。Y半抗原(Fuc.alpha1.fwdarw.2Gal.beta.1.fwdarw。4[Fuc.Alpha.1.fwdarw.3][GlcNAc]和聚合型X半抗原在小鼠胚胎癌细胞上的荧光激活流式细胞术检测。在移植后小鼠胚胎的石蜡切片中，在5.5-7.5天的胚胎外胚层和内脏内胚层中检测到Y半抗原，这种抗原表达模式与先前报道的X半抗原(SSEA-1)相同。因此，女孩：.alpha.1.fwdarw。2(H)和GlcNAc：α。.fwdarw。3(X)岩藻糖基转移酶在胚胎发育过程中似乎是共同调节的。然而，在植入前发育过程中，X和Y半抗原的表达发生了相反的变化。与X半抗原不同的是，Y半抗原在16-细胞囊胚、32-细胞和-囊胚上表达最多。然而，体外培养到囊胚期的八细胞胚胎没有获得Y半抗原，这表明子宫环境对糖类抗原的表达有作用。F9胚胎癌细胞的匀浆中含有一种有效的GlcNAc：α1.fwdarw。3岩藻糖基转移酶活性，以及较弱的Gal：α1.fwdarw。2以副糖苷为底物的岩藻糖基转移酶活力。结果表明，胚胎细胞表面碳水化合物表型代表了糖基转移酶之间对可用底物的竞争平衡。发育过程中碳水化合物表达的快速变化可能反映了细胞承诺和决定的中间状态，这对谱系形成和形态形成至关重要。
The X hapten (Gal.beta.1 .fwdarw. 4[Fuc.alpha.1 .fwdarw. 3]GlcNAc) may play an important role in the adhesion of blastomeres during compaction. Therefore, we have investigated more thoroughly developmental changes in the fucosylation of lactoseries carbohydrate chains and the enzymatic basis of these fucosylation changes using well-characterized monoclonal antibodies. The Y hapten (Fuc.alpha.1 .fwdarw. 2Gal.beta.1 .fwdarw. 4[Fuc.alpha.1 .fwdarw. 3]GlcNAc) and polymeric X haptens were detected by fluorescence-activated flow cytometry on murine embryonal carcinoma cells. In paraffin sections of postimplantation mouse embryos, the Y hapten was detected in the embryonic ectoderm and visceral endoderm on Days 5.5-7.5; this pattern of antigen expression is identical to that previously reported for the X hapten (SSEA-1). Thus, the Gal:.alpha.1 .fwdarw. 2 (H) and GlcNAc: .alpha. .fwdarw. 3 (X) fucosyltransferases appear to be co-regulated during embryogenesis. Reciprocal changes in X and Y hapten expression were observed, however, during preimplantation development. Unlike the X hapten, the Y hapten is expressed maximally on 16-cell morulae and 32- and 64-cell blastocysts. Eight-cell embryos cultured to the blastocyst stage in vitro did not acquire the Y hapten, however, suggesting a role for the uterine environment in carbohydrate antigen expression. Homogenates of F9 embryonal carcinoma cells were found to possess a potent GlcNAc:.alpha.1 .fwdarw. 3 fucosyltransferase activity, as well as a weaker Gal:.alpha.1 .fwdarw. 2 fucosyltransferase activity, using paragloboside as a substrate. The results suggest that embryonic cell surface carbohydrate phenotypes represent a balance in the competition between glycosyltransferases for available substrates. Rapid changes in carbohydrate expression during development may reflect intermediate states of cellular commitment and determination that are critical for lineage formation and morphogenesis.