Expression of peroxisome proliferator-activated receptor γ in renal cell carcinoma and growth inhibition by its agonists
Expression of peroxisome proliferator-activated receptor γ in renal cell carcinoma and growth inhibition by its agonists
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DOI：
10.1006/bbrc.2001.5640
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发表时间：
2001-09-28
影响因子：
3.1
通讯作者：
Sano, H
中科院分区：
文献类型：
--
作者：
Inoue, K;Kawahito, Y;Sano, H
过氧化物酶体增殖物激活受体-γ（PPAR-gamma）是属于类固醇受体超家族的配体激活的转录因子。它在脂肪细胞分化和肿瘤发生中起作用。目前，PPAR-gamma表达上调在多种不同的恶性肿瘤中频繁发生。在这项研究中，我们研究了PPAR-gamma在人肾细胞癌（RCC）组织中的表达，以及PPAR-gamma在人RCC衍生细胞系细胞生长中的作用。免疫组织化学显示，在所有载玻片从癌症标本的PPAR-gamma强免疫反应性表达。RT-PCR和Westernblot检测显示，3株肾癌细胞表达PPAR-gamma mRNA及其蛋白。MTT法显示合成的PPAR-γ激动剂噻唑烷二酮化合物（吡格列酮和曲格列酮）和内源性PPAR-γ配体15-脱氧-δ 12，14-前列腺素J（2）（15 dPGJ（2））抑制RCC细胞的生长。这些结果表明，PPAR-gamma可能成为RCC治疗的新靶点。(C)北京：科学出版社.
Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) is a ligand-activated transcriptional factor belonging to the steroid receptor superfamily. It plays a role in both adipocyte differentiation and tumorgenesis. Up-date, the up-regulation of PPAR-gamma expression is a frequent occurrence in a variety of different malignant tumors. In this study, we investigated the expression of PPAR-gamma in human renal cell carcinoma (RCC tissues, and the role of PPAR-gamma in cell growth in human RCC-derived cell lines. Immunohistochemistry showed a strong immunoreactive expression of PPAR-gamma in all slides from cancer specimens. RT-PCR and Western blot analysis showed 3 RCC cell lines expressed PPAR-gamma mRNA and its protein. MTT assay in 3 RCC cells showed that the synthetic PPAR-gamma agonists thiazolidinedione compounds (pioglitazone and troglitazone) and the endogeneous PPAR-gamma ligand, 15-deoxy-Delta 12,14-prostaglandin J(2) (15dPGJ(2)) inhibited the growth of the RCC cells. These results suggest that PPAR-gamma may become a new target in the treatment of RCC. (C) 2001 Academic Press.