Multiple-fungicide resistance to carbendazim, diethofencarb, procymidone, and pyrimethanil in field isolates of Botrytis cinerea from tomato in Henan Province, China
Multiple-fungicide resistance to carbendazim, diethofencarb, procymidone, and pyrimethanil in field isolates of Botrytis cinerea from tomato in Henan Province, China
复制标题
中国河南省番茄灰霉病田间分离株对多菌灵、乙霉威、腐霉利和嘧霉胺的多重抗药性
DOI：
10.1016/j.cropro.2016.02.012
复制
发表时间：
2016-06-01
期刊：
影响因子：
2.8
通讯作者：
Chen, Genqiang
中科院分区：
文献类型：
--
作者：
Liu, Shengming;Che, Zhiping;Chen, Genqiang
灰葡萄孢菌是引起番茄灰霉病的真菌病原体，经常表现出多种杀菌剂抗性。本研究测定了263株B菌对多菌灵、乙霉威、腐霉利和嘧霉胺的敏感性。2013年和2014年期间，中国河南省87个商业温室番茄植株的病果和叶片中的灰霉病。在供试菌株中，分别有89%、93%、85%和86%的菌株对Car、Die、Prc和Pyr具有抗性。12个表型被发现具有不同的敏感性模式，和多杀菌剂抗性被发现一个，两个，三个或四个杀菌剂。多重抗药性菌株（Car（R）Die（R）Prc（R）Pyr（R））的比例为68%。B的菌丝生长、产孢量和菌丝干重在抗性和敏感表型之间没有显著差异。灰霉病菌分离物但Car（S）Die（R）Prc（S）Pyr（S）菌株的毒力高于其他杀菌剂抗性表型菌株。所有的多菌灵和乙霉威耐药表型携带点突变密码子198（E198 A或E198 K）或200（F200 Y）在β-微管蛋白基因，和所有的腐霉利耐药表型携带点突变密码子369（Q369 P）和373（N373 S）在Bos 1基因。这些结果为B的抗性风险评估提供了重要的参考数据。结果表明，河南省番茄灰霉病菌对多菌灵、乙霉威、腐霉利和嘧霉胺的抗性频率很高，表明有必要对灰霉病进行综合防治。(C)2016爱思唯尔有限公司版权所有
Botrytis cinerea, the fungal pathogen responsible for causing gray mold in tomatoes, frequently exhibits multiple fungicide resistance. In this study, the sensitivity to carbendazim (Car), diethofencarb (Die), procymidone (Prc), and pyrimethanil (Pyr) was determined for 263 isolates of B. cinerea from diseased fruit and leaves of tomato plants from 87 commercial greenhouses in Henan Province of China during 2013 and 2014. Of the isolates tested, 89%, 93%, 85%, and 86% were resistant to Car, Die, Prc, and Pyr, respectively. Twelve phenotypes were found having different sensitivity patterns, and multi-fungicide resistance was found to one, two, three or four fungicides. The percentage of multiple fungicide resistant isolates (Car(R)Die(R)Prc(R)Pyr(R)) was 68%. Mycelial growth, spore production, and mycelial dry weight were not significantly different between resistant and sensitive phenotypes of the B. cinerea isolates. However, the virulence of Car(S)Die(R)Prc(S)Pyr(S) isolates was higher than that of the other fungicide resistance phenotypes. All of the carbendazim and diethofencarb resistant phenotypes carried a point mutation at codon 198 (E198A or E198K) or 200 (F200Y) in the beta-tubulin gene, and all of the procymidone resistant phenotypes carried point mutations at codons 369 (Q369P) and 373 (N373S) in the Bos1 gene. These results provided important reference data for assessment of resistance risk of B. cinerea isolates from commercial tomato transplants in Henan Province, indicating the existence of a high frequency of resistance to carbendazim, diethofencarb, procymidone, and pyrimethanil revealing the necessity of a more integrated control of gray mold. (C) 2016 Elsevier Ltd. All rights reserved.