Many cold sensitive peripheral neurons of the mouse do not express TRPM8 or TRPA1
Many cold sensitive peripheral neurons of the mouse do not express TRPM8 or TRPA1
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DOI：
10.1016/j.ceca.2006.07.008
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发表时间：
2007-04-01
期刊：
影响因子：
4
通讯作者：
Koltzenburg, Martin
中科院分区：
文献类型：
--
作者：
Munns, Clare;AlQatari, Mona;Koltzenburg, Martin
周围神经系统的神经元通过激活专门的离子通道来检测温度的变化。瞬时受体电位家族 TRPM8 和 TRPA1 的成员是冷刺激主要传感器的候选者。使用比率钙成像，我们现在显示 19% 的急性分离小鼠背根 (DRG) 和 45% 的颈上神经节 (SCG) 神经元对短暂的冷刺激有反应。在冷反应 DRG 神经元中，34 +/- 2% 对 TRPM8 激动剂薄荷醇有反应，18 +/- 3% 对 TRPA1 激动剂芥子油有反应，5% 对两种刺激都有反应。三分之一的冷敏感神经元对任何 TRP 通道激动剂没有反应。 SCG 的冷敏感神经元对薄荷醇没有反应，只有 3% 对芥末油有反应。 SCG 神经元的阈值温度明显低于 DRG 神经元的阈值。使用实时 PCR，TRPA1 在 DRG 中的表达量是 SCG 的 100 倍以上，而 TRPM8 仅存在于 DRG 中。 SCG 中相对少量的 TRPA1 转录本与高水平的冷敏感性无关。我们得出的结论是，交感神经元和很大一部分感觉神经元的冷敏感性是在 TRPM8 和 TRPA1 缺失的情况下产生的。 (c) 2006 Elsevier Ltd. 保留所有权利。
Neurons of the peripheral nervous system detect changes in temperature through activation of specialised ion channels. Members of the transient receptor potential family TRPM8 and TRPA1 are candidates for the principal transducers of cold stimuli. Using ratiometric calcium imaging we now show that 19% of acutely dissociated mouse dorsal root (DRG) and 45% of superior cervical ganglia(SCG) neurons responded to a brief cold stimulus. Amongst cold-responsive DRG neurons 34 +/- 2% responded to the TRPM8 agonist menthol, 18 +/- 3% to the TRPA1 agonist mustard oil and 5% to both stimuli. A third of the cold-sensitive neurons did not respond to any TRP channel agonist. Cold-sensitive neurons of the SCG did not respond to menthol and only 3% responded to mustard oil. The threshold of SCG neurons was at significantly cooler temperatures than that of DRG neurons. Using real-time PCR, TRPA1 was expressed over 100-fold more in DRG than SCG, while TRPM8 was present in DRG only. The relatively small amount of TRPA1 transcript present in SCG did not correlate with the high level of cold sensitivity. We conclude that cold sensitivity in sympathetic neurons and in a significant proportion of sensory neurons is generated in the absence of TRPM8 and TRPA1. (c) 2006 Elsevier Ltd. All rights reserved.