Peroxisome deficiency represses the expression of n-alkane-inducible YIALK1 encoding cytochrome P450ALK1 in Yarrowia lipolytica
Peroxisome deficiency represses the expression of n-alkane-inducible YIALK1 encoding cytochrome P450ALK1 in Yarrowia lipolytica
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DOI：
10.1016/s0378-1097(02)00838-8
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发表时间：
2002-08-27
影响因子：
2.1
通讯作者：
Ohta, A
中科院分区：
文献类型：
--
作者：
Sumita, T;Iida, T;Ohta, A
在编码正构烷烃同化酵母Yarrowia lipolytica的CYP 52家族的P450细胞色素的8个基因（YlALK 1-YlALK 8）中，YlALK 1最受具有短烃链的正构烷烃（例如正癸烷）的诱导，并且参与正构烷烃的初始羟基化。为了确定调控YlALK 1表达的因素，我们分离了正癸烷同化缺陷突变体BO-6-1，其YlALK 1表达水平低于野生型。通过互补BO-6-1的突变，我们克隆了一个具有1062 bp开放阅读框的基因。推测的基因产物是354个氨基酸的蛋白质，并且与其他生物的Pex 10 ps具有显著的同源性。我们将这个基因命名为YlPEX 10。YlPex 10 p在其C-末端区域具有Pex 10 p中常见的C3 HC 4环指基序。该基序对于YlPex 10 p的功能也是必需的。BO-6-1和YlPEX 10的无效突变体在碳源改变为正癸烷后均不能形成过氧化物酶体，并且显示YlALK 1的低水平转录。此外，YlPEX 5和YlPEX 6破坏物也显示VALKI的低水平转录，如YlPEX 10破坏物和BO-6-1突变体。我们建议，在这种生物过氧化物酶体缺乏抑制正烷烃诱导的YlALK 1编码细胞色素P450 ALK 1的表达。(C)2002年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Among the eight genes (YlALK1-YlALK8) encoding P450 cytochromes of the CYP52 family of the n-alkane-assimilating yeast Yarrowia lipolytica, YlALK1 is most highly induced by n-alkanes with short hydrocarbon chains, such as n-decane, and involved in the initial hydroxylation of n-alkane. To determine the factors regulating YlALK1 expression, we isolated an n-decane assimilation-deficient mutant, BO-6-1, whose YlALK1 expression level was lower than that of the wild-type. By complementation of the mutation of BO-6-1, we cloned a gene having an open reading frame of 1062 bp. The putative gene product is a protein of 354 amino acids and has significant homology to Pex10ps of other organisms. We named this gene YlPEX10. YlPex10p has a C3HC4 ring finger motif common among Pex10ps in its C-terminal region. This motif was also essential for the function of YlPex10p. Both BO-6-1 and a null mutant of YlPEX10 failed to form peroxisome and showed low-level transcription of YlALK1 after the change of carbon source to n-decane. Furthermore, YlPEX5 and YlPEX6 disruptants also showed low-level transcription of VALKI like the YlPEX10 disruptant and BO-6-1 mutant. We propose that in this organism peroxisome deficiency represses the expression of n-alkane-inducible YlALK1 encoding cytochrome P450ALK1. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.