Actin Polymerization and ESCRT Trigger Recruitment of the Fusogens Syntaxin-2 and EFF-1 to Promote Membrane Repair in C. elegans
Actin Polymerization and ESCRT Trigger Recruitment of the Fusogens Syntaxin-2 and EFF-1 to Promote Membrane Repair in C. elegans
复制标题
肌动蛋白聚合和 ESCRT 触发 Fusogens Syntaxin-2 和 EFF-1 的募集，以促进线虫的膜修复
DOI：
10.1016/j.devcel.2020.06.027
复制
发表时间：
2020-09-14
影响因子：
11.8
通讯作者：
Xu, Suhong
中科院分区：
文献类型：
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作者：
Meng, Xinan;Yang, Qingxian;Xu, Suhong
膜修复对于细胞和生物体的生存至关重要。胞吐作用和胞吞作用促进单个细胞内小伤口的膜修复;然而，尚不清楚在后生动物中质膜上的大伤口如何修复。在这里，我们表明，创伤触发快速转录上调和动态招聘的融合因子EFF-1的伤口部位在C。线虫表皮细胞EFF-1在受伤的膜上的募集依赖于肌动蛋白细胞骨架，并且对于膜修复是重要的。我们确定syntaxin-2（SYX-2）作为EFF-1募集的重要调节因子。SYX-2与EFF-1的C末端相互作用以促进其募集，从而促进内质膜和外质膜修复。此外，我们发现SYX-2-EFF-1修复机制作用于ESCRT III信号的下游。总之，我们的研究结果确定了膜修复的关键途径，并为损伤后的组织修复和再生医学提供了见解。
Membrane repair is essential for cell and organism survival. Exocytosis and endocytosis facilitate membrane repair in small wounds within a single cell; however, it remains unclear how large wounds in the plasma membrane are repaired in metazoans. Here, we show that wounding triggers rapid transcriptional upregulation and dynamic recruitment of the fusogen EFF-1 to the wound site in C. elegans epidermal cells. EFF-1 recruitment at the wounded membrane depends on the actin cytoskeleton and is important for membrane repair. We identified syntaxin-2 (SYX-2) as an essential regulator of EFF-1 recruitment. SYX-2 interacts with the C terminus of EFF-1 to promote its recruitment, facilitating both endoplasmic and exoplasmic membrane repair. Furthermore, we show that SYX-2-EFF-1 repair machinery acts downstream of the ESCRT III signal. Together, our findings identify a key pathway underlying membrane repair and provide insights into tissue repair and regenerative medicine after injury.