Immunofluorescent localization of immunoglobulins on the cell surface of mouse oocytes and preimplantation embryos.
Immunofluorescent localization of immunoglobulins on the cell surface of mouse oocytes and preimplantation embryos.
复制标题
免疫球蛋白在小鼠卵母细胞和植入前胚胎的细胞表面上的免疫荧光定位。
DOI：
10.1007/bf01133393
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Obasaju,MF
中科院分区：
文献类型：
--
作者：
Wiley,LM;Obasaju,MF
通过间接免疫荧光测定活体小鼠卵母细胞和植入前胚胎将异源血清蛋白从培养基吸附到细胞表面的能力。 IgG 类牛和人免疫球蛋白被卵母细胞和植入前胚胎的所有阶段吸附，而小鼠或山羊来源的 IgG 则不然。相反，没有血清白蛋白被吸附到小鼠卵母细胞和植入前胚胎的细胞表面。根据一些（但不是全部）所测试物种的 IgG 差异结合，我们得出结论，小鼠卵母细胞和植入前胚胎上的这些细胞表面 IgG“受体”本质上可能是嗜异性的。无论用于提供卵母细胞和胚胎的小鼠品系如何，都进行了类似的观察。这些观察结果提出了一个问题：是否也应该检测人类卵母细胞和植入前胚胎吸附动物血清蛋白的能力，动物血清蛋白可能作为人类体外受精/胚胎移植项目中使用的培养基中人血清的替代蛋白补充剂。
Living mouse oocytes and preimplantation embryos were assayed by indirect immunofluorescence for their ability to adsorb heterologous serum proteins from culture media to their cell surfaces. Bovine and human immunoglobulins of the IgG class were adsorbed by the oocytes and all stages of preimplantation embryos, while IgG of mouse or goat origin was not. In contrast none of the serum albumins was adsorbed to the cell surfaces of mouse oocytes and preimplantation embryos. From the differential binding of IgG of some, but not all, of the species that were tested, we concluded that these cell surface IgG “receptors” on mouse oocytes and preimplantation embryos are likely to be heterophilic in nature. Similar observations were made irrespective of the strain of mice used to provide the oocytes and embryos. These observations raise the question of whether human oocytes and preimplantation embryos should also be assayed for their ability to adsorb animal serum proteins that are possible candidates as a substitute protein supplement for human serum in culture medium used in human in vitro fertilization/embryo transfer programs.