Intravital bone imaging by two-photon excitation microscopy to identify osteocytic osteolysis in vivo
Intravital bone imaging by two-photon excitation microscopy to identify osteocytic osteolysis in vivo
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DOI：
10.1016/j.bone.2015.01.013
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发表时间：
2015-05-01
期刊：
影响因子：
4.1
通讯作者：
Ishii, Masaru
中科院分区：
文献类型：
--
作者：
Sano, Hiroshige;Kikuta, Junichi;Ishii, Masaru
骨是一种高度动态的器官，其中几种细胞类型协同发挥作用。其中，骨细胞最近成为骨稳态的重要调节者，尽管其调节机制尚不清楚。在这里，通过双光子激发显微镜进行的活体骨成像使我们能够直接观察骨细胞溶解或骨陷窝-小管系统中的骨吸收。骨细胞陷窝和小管网络在小鼠胫骨皮质成像在体内钙黄绿素染色，并在这些结构中的局部酸化监测使用局部应用的pH传感器。我们还证明坐骨神经切除术会导致骨细胞陷窝周围的显着酸化和陷窝小管区域的扩大。这些结果为骨细胞性骨溶解提供了强有力的证据，并表明双光子活体显微镜可用于分析这种现象。(c)2015 Elsevier Inc. All rights reserved.
Bone is a highly dynamic organ in which several cell types function cooperatively. Among these, osteocytes have recently emerged as an important regulator of bone homeostasis, although their mechanism of regulation is unclear. Here, intravital bone imaging by two-photon excitation microscopy allowed us to directly visualize osteocytic osteolysis', or resorption of bone in the lacuno-canalicular system. Osteocyte lacunae and the canalicular network in the cortex of murine tibiae were imaged by in vivo calcein staining, and local acidification in these structures was monitored using a topically applied pH sensor. We also demonstrated that sciatic neurectomy causes significant acidification around osteocytic lacunae and enlargement of lacuno-canalicular areas. These results provide strong evidence for osteocytic osteolysis, and demonstrate that two-photon intravital microscopy is useful for analysis of this phenomenon. (c) 2015 Elsevier Inc. All rights reserved.