Inhibitory effect of whole strawberries, garlic juice or kale juice on endogenous formation of N-nitrosodimethylamine in humans
Inhibitory effect of whole strawberries, garlic juice or kale juice on endogenous formation of N-nitrosodimethylamine in humans
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DOI：
10.1016/s0304-3835(02)00076-9
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发表时间：
2002-08-08
期刊：
影响因子：
9.7
通讯作者：
Sung, NJ
中科院分区：
文献类型：
--
作者：
Chung, MJ;Lee, SH;Sung, NJ
对草莓、大蒜和羽衣甘蓝提取物抑制亚硝化进行了体外和体内实验。草莓、大蒜和羽衣甘蓝提取物在体外抑制亚硝化。然而，大蒜提取物比草莓和羽衣甘蓝提取物具有更强的体外抑制化学亚硝化的能力。通过Prep-1-C将大蒜提取物的大蒜甲醇可溶级分分级为G1-G4级分。组分 G1 抑制 N-亚硝基二甲胺 (NDMA) 形成 84 +/- 1%。我们对 27 名男性和 13 名年龄为 24 +/- 3 岁的女性（每组 10 名健康志愿者），在服用硝酸盐（400 毫克/天）和富含胺的饮食后，在人体中形成致癌物 NDMA 进行了研究，并研究了通过服用整个草莓（300 克）、大蒜汁（200 克：饮用水中加入 75 克大蒜汁）或羽衣甘蓝汁（200 克）可能对致癌物质 NDMA 的形成的抑制作用。硝酸盐摄入导致平均唾液硝酸盐和亚硝酸盐浓度显着升高。此外，与对照日相比，实验日尿液中硝酸盐的排泄量显着增加。当在富含胺的饮食和硝酸盐后立即提供整个草莓、大蒜汁或羽衣甘蓝汁时，与摄入富含胺的饮食和硝酸盐后的 NDMA 排泄量相比，NDMA 排泄量分别减少 70%、71% 和 44%。这些结果表明，食用整个草莓、大蒜汁或羽衣甘蓝汁可以减少内源性 NDMA 的形成。 (C) 2002 年，爱思唯尔科学爱尔兰有限公司出版。
In vitro and in vivo experiments were performed on inhibition of nitrosation by strawberry, garlic, and kale extracts. Strawberry, garlic, and kale extracts inhibited nitrosation in vitro. However, garlic extract has a greater ability to inhibit the chemical nitrosation in vitro than strawberry and kale extracts. The garlic methanol-soluble fraction of the garlic extract was fractionated into G1-G4 fractions by Prep-l-C. Fraction G1 inhibited N-nitrosodimethylamine (NDMA) formation by 84 +/- 1%. We studied the formation of the carcinogen NDMA in humans after administration of nitrate (400 mg/day) in combination with an amine-rich diet and its possible inhibition by administration of whole strawberries (300 g), garlic juice (200 g: 75 g garlic juice in drinking water), or kale juice (200 g) in 27 males and 13 females (ten healthy volunteers in each group) of age 24 +/- 3 years. Nitrate intake resulted in a significant rise in mean salivary nitrate and nitrite concentrations. Also, nitrate excretion in urine during the experimental day was significantly increased compared with the control days. When whole strawberries, garlic juice, or kale juice was provided immediately after an amine-rich diet with a nitrate, NDMA excretion was decreased by 70, 71, and 44%, respectively, compared with NDMA excretion after ingestion of an amine-rich diet with a nitrate. These results suggest that consumption of whole strawberries, garlic juice, or kale juice can reduce endogenous NDMA formation. (C) 2002 Published by Elsevier Science Ireland Ltd.