Nucleic acid amplification-based techniques for pathogen detection and identification.

Nucleic acid amplification-based techniques for pathogen detection and identification.
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DOI:
10.1016/j.meegid.2005.08.004
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发表时间:
2006-01
期刊:
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
影响因子:
--
通讯作者:
Giglio S
Giglio S
中科院分区:
其他
文献类型:
--
作者:
Monis PT;Giglio S

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核酸扩增技术已经彻底改变了诊断和研究行业。目前能够实时检测扩增的技术正迅速成为行业标准,尤其是在诊断领域。在这篇综述中,我们描述和探讨了多种实时检测化学和扩增技术在病原体检测和鉴定中的应用,包括聚合酶链反应、核酸序列扩增、链位移扩增和连接酶链反应。新技术的出现,如片上实验室设备和光可切割连接器,也进行了讨论。
Nucleic acid amplification techniques have revolutionised diagnostic and research industries. Current technologies that allow the detection of amplification in real-time are fast becoming industry standards, particularly in a diagnostic context. In this review, we describe and explore the application of numerous real-time detection chemistries and amplification techniques for pathogen detection and identification, including the polymerase chain reaction, nucleic acid sequence-based amplification, strand displacement amplification and the ligase chain reaction. The emergence of newer technologies, such as lab-on-a-chip devices and photo-cleavable linkers, is also discussed.
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