Photosensitive MRI biosensor for BCRP-Targeted uptake and light-induced inhibition of tumor cells.
Photosensitive MRI biosensor for BCRP-Targeted uptake and light-induced inhibition of tumor cells.
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DOI：
10.1016/j.talanta.2021.122501
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发表时间：
2021-10
期刊：
影响因子：
6.1
通讯作者：
Long Zhao;Qianni Guo;Chen Yuan;Sha Li;Yaping Yuan;Qingbin Zeng;Xu Zhang;Chaohui Ye;Xin Zhou
中科院分区：
文献类型：
--
作者：
Long Zhao;Qianni Guo;Chen Yuan;Sha Li;Yaping Yuan;Qingbin Zeng;Xu Zhang;Chaohui Ye;Xin Zhou
核黄素及其衍生物是体内代谢中最重要的辅酶，与生命活动密切相关。本文将隐烷a与核黄素片段结合，研制了首个光敏129xe生物传感器，超极化129xe NMR/MRI技术记录了该传感器的光敏性，在水溶液中化学位移变化明显，为5.3 ppm。细胞荧光成像证实该生物传感器可在MCF-7细胞中富集，MTT实验证实辐照后细胞毒性增强。研究结果表明，该生物传感器在肿瘤靶向和光降解后抑制肿瘤细胞增殖方面具有潜在的应用前景。
Riboflavin and its derivatives are the most important coenzymes in vivo metabolism, and are closely related to life activities. In this paper, the first photolysis129Xe biosensor was developed by combining cryptophane-A with riboflavin moiety, which showed photosensitivity recorded by hyperpolarized129Xe NMR/MRI technology with an obvious chemical shift change of 5.3 ppm in aqueous solution. Cellular fluorescence imaging confirmed that the biosensor could be enriched in MCF-7 cells, and MTT assays confirmed that the cytotoxicity was enhanced after irradiation. Findings suggested that the biosensor has a potential application in tumor targeting and the inhibition of tumor cell proliferation after photodegradation.